Modeling microbial growth and biosynthesis of medium chain length polyhydroxyalkanoate (PHA) by Pseudomonas putida LS46
| dc.contributor.author | Sharifyazd, Shabnam | |
| dc.contributor.examiningcommittee | Hollaender, Hartmut (Civil Engineering) Cenkowski, Stefan (Biosystems Engineering) | en_US |
| dc.contributor.supervisor | Asadzadeh, Masoud (Civil Engineering) Levin, David (Biosystems Engineering) | en_US |
| dc.date.accessioned | 2020-06-26T19:28:44Z | |
| dc.date.available | 2020-06-26T19:28:44Z | |
| dc.date.copyright | 2020-06-20 | |
| dc.date.issued | 2020-06 | en_US |
| dc.date.submitted | 2020-06-21T01:46:52Z | en_US |
| dc.degree.discipline | Civil Engineering | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Among different types of thermoplastics, polyhydroxyalkanoates (PHAs) with different physical/mechanical and thermal properties are well known, as they are biodegradable, biocompatible, and non-toxic. However, the main drawback of PHA biosynthesis in large scale is the high cost of production. It is known that PHA production can be considerably increased under oxygen-limited conditions. In this study, growth and synthesis of medium chain length PHAs (mcl-PHAs) by Pseudomonas putida LS46 cultured with octanoic acid under oxygen-limited conditions in the batch mode was modeled. Four models including the Monod model incorporated Leudeking–Piret (MLP), the Moser model incorporated Leudeking–Piret (Moser-LP), the Logistic model incorporated Leudeking–Piret (LLP), and the Modified Logistic model incorporated Leudeking–Piret (MLLP) were investigated. Kinetic parameters of each model were calibrated by using the multi-objective optimization algorithm, Pareto Archived Dynamically Dimensioned Search (PA-DDS) by minimizing the sum of absolute error (SAE) for PHA production and growth simultaneously. Among the four models, MLP and Moser-LP models adequately represented the experimental data for oxygen limited conditions. In addition, the MLP and Moser-LP models could not adequately simulate PHA production under oxygen excess conditions. For growth, under oxygen excess conditions, this deviation was not very significant. | en_US |
| dc.description.note | October 2020 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/34729 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Polyhydroxyalkanoates, Batch kinetic modeling, Pseudomonas putida, Dissolved oxygen, Bioreactor, Multi-objective optimization | en_US |
| dc.title | Modeling microbial growth and biosynthesis of medium chain length polyhydroxyalkanoate (PHA) by Pseudomonas putida LS46 | en_US |
| dc.type | master thesis | en_US |