Satellite cell activation in adult zebrafish (Danio rerio) single muscle fibre cultures

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dc.contributor.authorZhang, Helia (Haoyue)
dc.contributor.examiningcommitteeAnderson, Gary (Biological Sciences) Leiter, Jeff(Surgery)en_US
dc.contributor.supervisorAnderson, Judy(Biological Sciences)en_US
dc.date.accessioned2013-07-30T20:24:52Z
dc.date.available2013-07-30T20:24:52Z
dc.date.issued2013-07-30
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractSatellite cells (SCs) are muscle stem cells that stay in a metabolically and mitotically quiescent state in adult skeletal muscle until activated. In mammals, SCs are activated and enter into the cell cycle for growth and regeneration. The mechanism initiating SC activation in vivo and in vitro, mediated by nitric oxide (NO) and hepatocyte growth factor (HGF) is described in the mouse model, but not in other species. Here, we assessed SC activation by counting bromodeoxyuridine (BrdU)-immuno-positive cells, and found that SC activation in zebrafish single muscle-fibre cultures is also NO and HGF dependent, peaking at 1 mM isosorbide dinitrate (ISDN, an NO donor drug) and 10 ng/mL HGF respectively, using dose-response experiments. Moreover, HGF signalling via the c-Met receptor is involved in the SC activation pathway and is considerably affected by temperature (i.e., 21 °C). Overall, understanding NO-HGF-c-Met signalling in SC activation gives new insights on fish muscle growth and conservation of regulatory pathways between species.en_US
dc.description.noteOctober 2013en_US
dc.identifier.urihttp://hdl.handle.net/1993/21970
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectsatellite cellen_US
dc.subjectactivationen_US
dc.subjectmuscle fibreen_US
dc.subjectzebrafishen_US
dc.titleSatellite cell activation in adult zebrafish (Danio rerio) single muscle fibre culturesen_US
dc.typemaster thesisen_US
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