The impact of vitamin D on innate immune responsiveness to pattern recognition receptor stimulation in humans

Simple item page
dc.contributor.authorFitch, Natascha
dc.contributor.examiningcommitteeBecker, Allan (Immunology) Mookherjee, Neeloffer (Immunology) Taylor, Carla (Human Nutritional Sciences)en_US
dc.contributor.supervisorHayGlass, Kent (Immunology)en_US
dc.date.accessioned2013-08-19T14:29:48Z
dc.date.available2013-08-19T14:29:48Z
dc.date.issued2013-08-19
dc.degree.disciplineImmunologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractObjective: Study the effects of vitamin D on viral driven innate immune responses, by looking at differences in cytokine production, receptor expression, and endogenous vitamin D levels. Methods: Primary peripheral blood mononuclear cells (PBMC) and epithelial cells (EC) were cultured in the presence of viral ligands and vitamin D. Enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR) were used to determine cytokine production and mRNA expression. Results: PBMC stimulated with toll-like receptor 4 ligand (TLR4L), but not viral TLR8L, led to decreased pro- and anti-inflammatory cytokine production in the presence of 1,25(OH)2D3. RIG-like receptor (RLR) activation, on the other hand, in primary EC exhibited decreased pro-inflammatory cytokine production in the presence of vitamin D. Conclusions: Our findings are among the first to show differences between bacterial and viral driven innate immune responses in the presence of vitamin D. As responsiveness in RLR activated primary EC was altered in the presence of vitamin D, our data reveal the importance of studying the immune system as a whole.en_US
dc.description.noteOctober 2013en_US
dc.identifier.urihttp://hdl.handle.net/1993/22036
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectVitamin Den_US
dc.subjectImmunologyen_US
dc.subjectCytokinesen_US
dc.subjectInflammationen_US
dc.titleThe impact of vitamin D on innate immune responsiveness to pattern recognition receptor stimulation in humansen_US
dc.typemaster thesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
natascha_fitch.pdf.pdf
Size:
1.38 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.25 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
FGS - Electronic Theses and Practica