Alteration of 11-Hydroxysteroid Dehydrogenase Type 1 and Glucocorticoid Receptor by Ethanol in Rat Liver and Mouse Hepatoma Cells

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dc.contributor.authorMeng, Zhaojie
dc.contributor.authorBao, Xueying
dc.contributor.authorZhang, Ming
dc.contributor.authorWei, Shengnan
dc.contributor.authorChang, Wenguang
dc.contributor.authorLi, Jing
dc.contributor.authorChen, Li
dc.contributor.authorNyomba, B. L. Grégoire
dc.date.accessioned2014-08-14T06:48:37Z
dc.date.available2014-08-14T06:48:37Z
dc.date.issued2013-6-1
dc.date.updated2014-08-14T06:48:37Z
dc.description.abstractAlcohol is a potential risk factor of type 2 diabetes, but its underlying mechanism is unclear. To explore this issue, Wistar rats and mouse hepatoma cells (Hepa 1–6) were exposed to ethanol, 8 g·kg−1·d−1 for 3 months and 100 mM for 48 h, respectively. Glucose and insulin tolerance tests in vivo were performed, and protein levels of 11-hydroxysteroid dehydrogenase type 1 (11-HSD1) and glucocorticoid receptor (GR) in liver and Hepa 1–6 cells were measured. Alterations of key enzymes of gluconeogenesis phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6 phosphatase (G6Pase), as well as glycogen synthase kinase 3a (GSK3α), were also examined. The results revealed that glucose levels were increased, and insulin sensitivity was impaired accompanied with liver injury in rats exposed to ethanol compared with controls. The 11-HSD1, GR, PEPCK, G6Pase, and GSK3α proteins were increased in the liver of rats treated with ethanol compared with controls. Ethanol-exposed Hepa 1–6 cells also showed higher expression of 11-HSD1, GR, PEPCK, G6Pase, and GSK3α proteins than control cells. After treatment of Hepa 1–6 cells exposed to ethanol with the GR inhibitor RU486, the expression of 11-HSD1 and GR was significantly decreased. At the same time the increases in PEPCK, G6Pase, and GSK3α levels induced by ethanol in Hepa 1–6 cells were also attenuated by RU486. The results indicate that ethanol causes glucose intolerance by increasing hepatic expression of 11-HSD1 and GR, which leads to increased expression of gluconeogenic and glycogenolytic enzymes.
dc.description.versionPeer Reviewed
dc.identifier.citationZhaojie Meng, Xueying Bao, Ming Zhang, et al., “Alteration of 11-Hydroxysteroid Dehydrogenase Type 1 and Glucocorticoid Receptor by Ethanol in Rat Liver and Mouse Hepatoma Cells,” Journal of Diabetes Research, vol. 2013, Article ID 218102, 8 pages, 2013. doi:10.1155/2013/218102
dc.identifier.doihttp://dx.doi.org/10.1155/2013/218102
dc.identifier.urihttp://hdl.handle.net/1993/23773
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderCopyright © 2013 Zhaojie Meng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleAlteration of 11-Hydroxysteroid Dehydrogenase Type 1 and Glucocorticoid Receptor by Ethanol in Rat Liver and Mouse Hepatoma Cells
dc.typeJournal Article
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