Inhibition of Intestinal α-Glucosidase and Glucose Absorption by Feruloylated Arabinoxylan Mono- and Oligosaccharides from Corn Bran and Wheat Aleurone

Simple item page
dc.contributor.authorMalunga, Lovemore Nkhata
dc.contributor.authorEck, Peter
dc.contributor.authorBeta, Trust
dc.date.accessioned2016-05-11T15:05:17Z
dc.date.available2016-05-11T15:05:17Z
dc.date.issued2016-3-17
dc.date.updated2016-05-08T09:32:09Z
dc.description.abstractThe effect of feruloylated arabinoxylan mono- and oligosaccharides (FAXmo) on mammalian α-glucosidase and glucose transporters was investigated using human Caco-2 cells, rat intestinal acetone powder, and Xenopus laevis oocytes. The isolated FAXmo from wheat aleurone and corn bran were identified to have degree of polymerization (DP) of 4 and 1, respectively, by HPLC-MS. Both FAXmo extracts were effective inhibitors of sucrase and maltase functions of the α-glucosidase. The IC50 for FAXmo extracts on Caco-2 cells and rat intestinal α-glucosidase was 1.03–1.65 mg/mL and 2.6–6.5 mg/mL, respectively. Similarly, glucose uptake in Caco-2 cells was inhibited up to 40%. The inhibitory effect of FAXmo was dependent on their ferulic acid (FA) content ( = 0.95). Sodium independent glucose transporter 2 (GLUT2) activity was completely inhibited by FAXmo in oocytes injected to express GLUT2. Our results suggest that ferulic acid and feruloylated arabinoxylan mono-/oligosaccharides have potential for use in diabetes management.
dc.description.versionPeer Reviewed
dc.identifier.citationLovemore Nkhata Malunga, Peter Eck, and Trust Beta, “Inhibition of Intestinal α-Glucosidase and Glucose Absorption by Feruloylated Arabinoxylan Mono- and Oligosaccharides from Corn Bran and Wheat Aleurone,” Journal of Nutrition and Metabolism, vol. 2016, Article ID 1932532, 9 pages, 2016. doi:10.1155/2016/1932532
dc.identifier.urihttp://dx.doi.org/10.1155/2016/1932532
dc.identifier.urihttp://hdl.handle.net/1993/31292
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderCopyright © 2016 Lovemore Nkhata Malunga et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleInhibition of Intestinal α-Glucosidase and Glucose Absorption by Feruloylated Arabinoxylan Mono- and Oligosaccharides from Corn Bran and Wheat Aleurone
dc.typeJournal Article
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
JNME.2016.1932532.xml
Size:
6.01 KB
Format:
Extensible Markup Language
Description:
Download
Thumbnail Image
Name:
JNME.2016.1932532.pdf
Size:
1.4 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.24 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
University of Manitoba Scholarship
Faculty of Agricultural and Food Sciences Scholarly Works