Characterisation of monolysocardiolipin acyltransferase activity
| dc.contributor.author | Ma, Brian Jason Lap-Yen | en_US |
| dc.date.accessioned | 2007-07-12T17:51:52Z | |
| dc.date.available | 2007-07-12T17:51:52Z | |
| dc.date.issued | 2000-07-01T00:00:00Z | en_US |
| dc.degree.discipline | Pharmacology and Therapeutics | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Cardiolipin (CL) is a unique phospholipid synthesised exclusively in the mitochondria where it is also mainly localised. Monolyso-CL acyltransferase is the enzyme responsible for re-acylating monolyso-CL to CL once a phospholipase A has removed one of the fatty acid tails. Because of CL's role in activating enzymes involved in energy metabolism, we investigated the effect of insulin on monolyso-CL acyltransferase activity and the composition of CL acyl groups. We hypothesised that some of the alterations in mitochondrial function may be due to the alteration in CL remodelling activity. Sprague-Dawley (SD) rats were treated with insulin (i.p. 12 U/rat) for 5 or 26 days. The hearts were then isolated and the mitochondrial fractions were collected. These mitochondria were assayed for monolyso-CL acyltransferase activity. In addition, streptozotocin-induced, diabetic SD rats were injected with saline for 5 days and the heart mitochondrial monolyso-CL acyltransferase activity was measured. The CL molecular species was examined. The characteristics of monolyso-CL acyltransferase activity from rat heart mitochondria were studied. In other experiments, the activity of monolyso-CL acyltransferase was localised using rat liver mitochondria. (Abstract shortened by UMI.) | en_US |
| dc.format.extent | 4223239 bytes | |
| dc.format.extent | 184 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.format.mimetype | text/plain | |
| dc.identifier.uri | http://hdl.handle.net/1993/2636 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.title | Characterisation of monolysocardiolipin acyltransferase activity | en_US |
| dc.type | master thesis | en_US |