MiRacles for babies with pulmonary hypoplasia: the effects of miR-10a and miR-200b on lung development
| dc.contributor.author | Visser, Robin | |
| dc.contributor.examiningcommittee | Boyd, April (Surgery) Pathak, Alok (Surgery) Ghavami, Saied (Human Anatomy and Cell Science) | en_US |
| dc.contributor.supervisor | Keijzer, Richard (Surgery) | en_US |
| dc.date.accessioned | 2016-01-14T15:01:33Z | |
| dc.date.available | 2016-01-14T15:01:33Z | |
| dc.date.issued | 2015 | |
| dc.degree.discipline | Surgery | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | INTRODUCTION: Pulmonary hypoplasia causes high morbidity and mortality in congenital diaphragmatic hernia (CDH) patients. MiR-10a and miR-200b are overexpressed in human CDH lungs. We aimed to define their roles in lung development. METHODS: We profiled miR-10a expression with RT-qPCR and in situ hybridization using a nitrofen rat model for CDH. The effects of miR-10a on airway branching were evaluated in lung explants. MiR-200b’s role in airway branching was assessed in miR-200b knockout lung explants. Crossing miR-200b knockout mice with CFP-E-Cadherin was used to evaluate miR-200b’s effects on epithelial differentiation. RESULTS: Expression of miR-10a was altered in the nitrofen model and miR-10a mimics reversed lung hypoplasia in vitro. Heterozygous miR-200b lung explants displayed reduced airway branching. CFP-E-Cadherin/miR-200b knockout lung explants showed reduced epithelial expression. CONCLUSION: Both miR-10a and miR-200b are critical for lung development and CDH. Normalizing their expression may reverse lung hypoplasia and reduce the associated morbidity and mortality in CDH. | en_US |
| dc.description.note | February 2016 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/31060 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | congenital diaphragmatic hernia | en_US |
| dc.subject | lung development | en_US |
| dc.subject | miR-10a | en_US |
| dc.subject | miR-200b | en_US |
| dc.subject | pulmonary hypoplasia | en_US |
| dc.title | MiRacles for babies with pulmonary hypoplasia: the effects of miR-10a and miR-200b on lung development | en_US |
| dc.type | master thesis | en_US |