Ebola virus nucleocapsid-like particles demonstrate consistent assembly, but a wide range of helical condensation

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dc.contributor.authorRabb, Melissa
dc.contributor.examiningcommitteeCoombs, Kevin (Medical Microbiology and Infectious Diseases) Mark, Brian (Microbiology) Wille, Holger (University of Alberta)en_US
dc.contributor.guestmembersBeniac, Daniel (Public Health Agency of Canada)en_US
dc.contributor.supervisorBooth, Timothy (Medical Microbiology and Infectious Diseases)en_US
dc.date.accessioned2017-09-13T15:29:48Z
dc.date.available2017-09-13T15:29:48Z
dc.date.issued2017
dc.degree.disciplineMedical Microbiology and Infectious Diseasesen_US
dc.degree.levelDoctor of Philosophy (Ph.D.)en_US
dc.description.abstractThe overall goal of this thesis was to determine whether envelope-free Ebola virus (EBOV) nucleocapsids could be isolated. We identified novel structures from EBOV infected cells that we have termed “proto-nucleocapsids”. These proto-nucleocapsids were ~30.5 nm in diameter and single particle image analysis revealed a hollow structure with small lobes, attributed to individual NP molecules. In addition, no large external protuberances on this structure were observed. As such, we hypothesize that the proto-nucleocapsids are the inner NP-RNA layer of the EBOV nucleocapsid. Expression of EBOV NP, VP24, VP30, VP35, and VP40 followed by density gradient ultracentrifugation of cell lysates enabled the isolation of novel nucleocapsid-like particles. These particles had a similar diameter to the EBOV nucleocapsid, but had a slightly larger helical pitch. Characterization of the nucleocapsid-like particles under various conditions demonstrated that the overall integrity of the helical structure was maintained, although a wide range of helical condensation was observed.en_US
dc.description.noteOctober 2017en_US
dc.identifier.urihttp://hdl.handle.net/1993/32595
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectEbolaen_US
dc.titleEbola virus nucleocapsid-like particles demonstrate consistent assembly, but a wide range of helical condensationen_US
dc.typedoctoral thesisen_US
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