Impact of Lactic Acid Bacteria on Dendritic Cells from Allergic Patients in an Experimental Model of Intestinal Epithelium

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dc.contributor.authorRatajczak, Céline
dc.contributor.authorDuez, Catherine
dc.contributor.authorGrangette, Corinne
dc.contributor.authorPochard, Pierre
dc.contributor.authorTonnel, André-Bernard
dc.contributor.authorPestel, Joël
dc.date.accessioned2014-08-14T07:05:43Z
dc.date.available2014-08-14T07:05:43Z
dc.date.issued2007-2-28
dc.date.updated2014-08-14T07:05:43Z
dc.description.abstractLactic acid bacteria (LAB) are Gram positive nonpathogenic commensal organisms present in human gastrointestinal tract. In vivo, LAB are separated from antigen-presenting cells such as dendritic cells (DC) by the intestinal epithelial barrier. In this study, the impact of one LAB strain (Lactobacillus casei ATCC393) on human monocyte-derived DC from allergic and healthy donors was assessed by using a polarized epithelium model. Confocal and flow cytometer analyses showed that immature DC efficiently captured FITC-labelled L. casei through the epithelial layer. After interaction with L. casei, DC acquired a partial maturation status (i.e., CD86 and CD54 increase) and increased their interleukin (IL)-10 and IL-12 production. Interestingly, after activation by L. casei in the presence of experimental epithelium, DC from allergic patients instructed autologous naïve CD4+ T cells to produce more interferon-γ than without the epithelium. Thus by modulating human DC reactivity, LAB and intestinal epithelium might modify T cell immune response and regulate the development of allergic reaction.
dc.description.versionPeer Reviewed
dc.identifier.citationCéline Ratajczak, Catherine Duez, Corinne Grangette, Pierre Pochard, André-Bernard Tonnel, and Joël Pestel, “Impact of Lactic Acid Bacteria on Dendritic Cells from Allergic Patients in an Experimental Model of Intestinal Epithelium,” Journal of Biomedicine and Biotechnology, vol. 2007, Article ID 71921, 9 pages, 2007. doi:10.1155/2007/71921
dc.identifier.doihttp://dx.doi.org/10.1155/2007/71921
dc.identifier.urihttp://hdl.handle.net/1993/23783
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderCopyright © 2007 Céline Ratajczak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleImpact of Lactic Acid Bacteria on Dendritic Cells from Allergic Patients in an Experimental Model of Intestinal Epithelium
dc.typeJournal Article
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