Incidence and molecular detection of yellows-type disease in carrots, associated with leafhoppers in southern Manitoba, Canada

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dc.contributor.authorWally, O
dc.contributor.authorDaayf, F
dc.contributor.authorKhadhair, AH
dc.contributor.authorAdam, L
dc.contributor.authorElliott, B
dc.contributor.authorShinners-Carnelley, T
dc.contributor.authorIranpour, M
dc.contributor.authorNorthover, P
dc.contributor.authorKeyworth, S
dc.date.accessioned2007-10-09T12:38:02Z
dc.date.available2007-10-09T12:38:02Z
dc.date.issued2004-12-31
dc.description.abstractAster yellows (AY), a disease caused by phytoplasmas, is a limiting factor to carrot production in Manitoba, Canada. The pathogen is transmitted by insect vectors, Macrosteles fascifrons (leafhoppers), and causes serious losses in many other crops. Traditional methods for estimating AY incidence in carrots and the proportion of AY-vector insects are based on visual symptoms and on bioassays of leafhopper populations. In the present study, techniques based on polymerase chain reaction (PCR) were adapted for the examination of insect and carrot tissues. A more accurate determination of infected plants and of virulent leafhoppers was achieved over two growing seasons. Surveys were carried out in five carrot fields in southern Manitoba throughout the 2001 and 2002 growing seasons. Large numbers of leafhoppers were captured weekly, and both direct and nested PCR were performed on individual samples in a population of more than 1000 insects and on 250 carrot samples as well as on several potential hosts around the carrot fields. According to PCR testing, visual assessments appear to overestimate the true incidence of AY in carrots. The percentage of leafhoppers carrying phytoplasma differed between the two years (18% and 31% in 2001 and 2002, respectively). However, higher proportions of leafhoppers carrying phytoplasmas were found early in the growing season in both study years. Therefore, implementation of management decisions would be more valuable early in the season, when the presence of the first leafhoppers is confirmed.en
dc.format.extent147341 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citation0706-0661; CAN J PLANT PATHOL, OCT-DEC 2004, vol. 26, no. 4, p.498 to 505.en
dc.identifier.doihttp://dx.doi.org/10.1080/07060660409507170
dc.identifier.urihttp://hdl.handle.net/1993/2907
dc.language.isoengen_US
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dc.rightsopen accessen_US
dc.statusPeer revieweden
dc.subjectaster yellowen
dc.subjectphytoplasmaen
dc.subjectmolecular detectionen
dc.subjectincidenceen
dc.subjectcarroten
dc.subjectMacrosteles fascifronsen
dc.subjectleafhopperen
dc.subjectinsect vectoren
dc.subjectWITCHES-BROOM PHYTOPLASMAen
dc.subjectPATHOGENIC MYCOPLASMALIKE ORGANISMSen
dc.subjectPURPLE CONEFLOWERen
dc.subjectHOST-PLANTen
dc.subjectRNA GENEen
dc.subject16S RDNAen
dc.subjectPCRen
dc.subjectIDENTIFICATIONen
dc.subjectAMPLIFICATIONen
dc.subjectRFLPen
dc.titleIncidence and molecular detection of yellows-type disease in carrots, associated with leafhoppers in southern Manitoba, Canadaen
dc.typejournal articleen_US
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