Cellular control of nuclear import of HIV
| dc.contributor.author | Rastogi, Ruchi | en_US |
| dc.date.accessioned | 2007-07-12T19:41:42Z | |
| dc.date.available | 2007-07-12T19:41:42Z | |
| dc.date.issued | 2001-08-22T00:00:00Z | en_US |
| dc.degree.discipline | Medical Microbiology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | To further define the relationship between T cell activation and the nuclear import of HIV pre-integration complex (PIC), this thesis examines the role of topoisomerase II (topoII), and enzyme induced and involved in a major remodeling of nucleus during T cell activation. The negatively selected highly purified resting CD4+ human T cells, free of monocytes, were infected with HIVIIIB and stimulated with a combination of anti-CD3 and anti-CD28 monoclonal antibodies (mAbs) in the presence or absence of different topoII inhibitors (VM-26, mAMSA, novobiocin, ICRF 187) that act through different mechanisms and block the activities of both topoII_ and topoIIB isozymes. Specific mRNA expression was monitored by RT-PCR, IL-2 production by ELISA and the expression of IL-2R_ by flow cytometry. Fully reverse-transcribed viral genome was monitored by PCR amplification and the nuclear migration of PIC was monitored by semi-quantitative PCR assays of 2-LTR circles. The entry of cells into S-phase was monitored by 3H-thymidine incorporation. (Abstract shortened by UMI.) | en_US |
| dc.format.extent | 3916961 bytes | |
| dc.format.extent | 184 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.format.mimetype | text/plain | |
| dc.identifier.uri | http://hdl.handle.net/1993/2672 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.title | Cellular control of nuclear import of HIV | en_US |
| dc.type | master thesis | en_US |