A microflow cytometer with simultaneous dielectrophoretic actuation for the optical assay and capacitive cytometry of individual fluid suspended bioparticles

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dc.contributor.authorRomanuik, Sean
dc.contributor.examiningcommitteeBridges, Gregory (Electrical and Computer Engineering) Lin, Francis (Physics and Astronomy)en
dc.contributor.supervisorThomson, Douglas (Electrical and Computer Engineering)en
dc.date.accessioned2009-09-14T21:24:54Z
dc.date.available2009-09-14T21:24:54Z
dc.date.issued2009-09-14T21:24:54Z
dc.degree.disciplineElectrical and Computer Engineeringen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractFluid suspended biological particles (bioparticles) flowing through a non-uniform electric field are actuated by the induced dielectrophoretic (DEP) force, known to be dependent upon the bioparticles’ dielectric phenotypes. In this work: a 10-1000 kHz DEP actuation potential applied to a co-planar microelectrode array (MEA) induces a DEP force, altering passing bioparticle trajectories as monitored using: (1) an optical assay, in which the lateral bioparticle velocities are estimated from digital video; and (2) a capacitive cytometer, in which a 1.478 GHz capacitance sensor measures the MEA capacitance perturbations induced by passing bioparticles, which is sensitive to the bioparticles’ elevations. The experimentally observed and simulated lateral velocity profiles of actuated polystyrene microspheres (PSS) and viable and heat shocked Saccharomyces cerevisiae cells verify that the bioparticles’ dielectric phenotypes can be inferred from the resultant trajectories due to the balance between the DEP force and the viscous fluid drag force.en
dc.description.noteOctober 2009en
dc.format.extent12090925 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citationFerrier, G.A., A.N. Hladio, D.J. Thomson, G.E. Bridges, M. Hedayatipoor, S. Olson, and M.R. Freeman (2007). Microfluidic electromanipulation with capacitive detection for cell diagnositc applications, Proc. NSTI 2:214-217.en
dc.identifier.citationFerrier, G.A., S.F. Romanuik, D.J. Thomson, G.E. Bridges, and M.R. Freeman (2008). Capacitance signatures for rapid detection of the polarity of the dielectrophoretic force on single yeast cells, Proc. NSTI 2:589-592.en
dc.identifier.citationFerrier, G.A., A.N. Hladio, D.J. Thomson, G.E. Bridges, M. Hedayatipoor, S. Olson, and M.R. Freeman (2008). Microfluidic electromanipulation with capacitive detection for the mechanical analysis of cells, Biomicrofluidics 2(4-044102):1-13.en
dc.identifier.citationFerrier, G.A., S.F. Romanuik, D.J. Thomson, G.E. Bridges, and M.R. Freeman (Submitted 2009). A microwave interferometric system for simultaneous actuation and detection of single biological cells, Lab on a Chip.en
dc.identifier.citationFerrier, G.A., S.F. Romanuik, D.J. Thomson, G.E. Bridges, and M.R. Freeman (Submitted 2009). Rapid classification of biological cells based on dielectrophoretic actuation with simultaneous capacitive detection, Proc. micro-TAS.en
dc.identifier.citationNikolic-Jaric, M., S.F. Romanuik, G.A. Ferrier, G.E. Bridges, M. Butler, K. Sunley, D.J. Thomson, and M.R. Freeman (2009). Microwave frequency sensor for detection of biological cells in microfluidic channels, Biomicrofluidics 3(3-034103):1-15.en
dc.identifier.citationRomanuik, S.F., G.A. Ferrier, D.J. Thomson, G.E. Bridges, S. Olson, and M.R. Freeman (2008). All-electronic detection and actuation of single biological cells for lab-on-a-chip applications, Proc. IEEE Sensors 1:634-637.en
dc.identifier.citationRomanuik, S.F., G.A. Ferrier, M.N. Jaric, D.J. Thomson, G.E. Bridges, and M.R. Freeman (Submitted 2009). Dielectrophoretic actuation and simultaneous detection of individual bioparticles, Advances in Biomedical Sensing, Measurements, Instrumentation, and Systems, Eds. A. Lay-Ekuakille and S.C. Mukhopadhyay, Springer – Verlag.en
dc.identifier.urihttp://hdl.handle.net/1993/3205
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectMicroflowen
dc.subjectMicrofluidicen
dc.subjectCytometeren
dc.subjectCytometryen
dc.subjectDielectrophoreticen
dc.subjectDielectrophoresisen
dc.subjectInterferometeren
dc.subjectInterferometricen
dc.subjectCapacitive Sensoren
dc.subjectCapacitance Sensingen
dc.subjectCapacitive Detectoren
dc.subjectCapacitance Detectionen
dc.subjectPolystyreneen
dc.subjectOptical Assayen
dc.subjectElectrokineticen
dc.subjectActuationen
dc.subjectYeasten
dc.subjectSaccharomyces cerevisiaeen
dc.subjectSingle-cell Diagnosticen
dc.subjectSingle-cell Diagnosisen
dc.subjectDielectric Modelingen
dc.subjectCOMSOLen
dc.subjectTrackeren
dc.subjectTrajectoryen
dc.subjectVelocity Profileen
dc.subjectCapacitive Signatureen
dc.subjectCapacitance Signatureen
dc.subjectMicroelectrodeen
dc.titleA microflow cytometer with simultaneous dielectrophoretic actuation for the optical assay and capacitive cytometry of individual fluid suspended bioparticlesen
dc.typemaster thesisen_US
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