Fate of estrogenic compounds in agricultural soils and development of an immunoassay for their environmental detection
| dc.contributor.author | Caron, Emmanuelle | |
| dc.contributor.examiningcommittee | Hanson, Mark (Environmental Geography) Flaten, Don (Soil Science) Topp, Edward (Agriculture and Agri-Food Canada) | en |
| dc.contributor.supervisor | Farenhorst, Annemieke (Soil Science) Sheedy, Claudia (Soil Science) | en |
| dc.date.accessioned | 2011-06-24T19:15:18Z | |
| dc.date.available | 2011-06-24T19:15:18Z | |
| dc.date.issued | 2011-06-24T19:15:18Z | |
| dc.degree.discipline | Soil Science | en_US |
| dc.degree.level | Doctor of Philosophy (Ph.D.) | en_US |
| dc.description.abstract | Estrogens produced by livestock can be released into soils when their manure is spread onto agricultural land. This is the first study to determine the sorption of a range of estrogens in a wide range of soils at the regional scale, including the sorption of the phytoestrogen equol which had never been previously studied. Sorption increased in the order of 17β-estradiol=estriol <estrone<equol in surface soils collected from 41 agricultural fields in Alberta and was significantly positively correlated with soil organic carbon content (SOC) for all estrogens. 17β-estradiol was further investigated and its mineralization in non-amended and manure-amended soils never exceeded 30% at 90 days, which suggest that even under optimum environmental conditions for mineralization, 17β-estradiol or its metabolites estrone and/or estriol appear to have a relatively long persistence in Alberta soils. Maximum 17β-estradiol mineralization was significantly positively correlated with sorption and hence increased in soils with greater SOC such as those used in this study with a long-term history of solid beef manure applications. Two ELISAs were developed using rabbit polyclonal antibodies for future field experiments and environmental monitoring. Of these, a developed 17β-estradiol+estrone+estriol ELISA could detect estriol in water from an edge of field experiment at concentrations as low as 1 ng mL-1. | en |
| dc.description.note | October 2011 | en |
| dc.format.extent | 2221561 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.citation | Caron, E., Farenhorst, A., Mc Queen, R., Sheedy, C., Goddard, T.and Gaultier, J. 2010. Mineralization of 17ß-estradiol in 36 Surface Soils from Alberta, Canada. Agriculture, Ecosystems and Environment, 139(4):534-545 | en |
| dc.identifier.citation | Caron E., Sheedy, C. and Farenhorst, A. 2010. Development of Competitive ELISAs for 17β-Estradiol and 17β-Estradiol +Estrone+Estriol using rabbit polyclonal antibodies. Journal of Environmental Science and Health Part B: Pesticides, food contaminants and agricultural wastes 45(2):145-151 | en |
| dc.identifier.citation | Caron, E., Farenhorst, A., Gaultier, J., Rank, N., Goddard, T. and Sheedy, C. 2010. Sorption of Four Estrogens by Surface Soils from 41 Cultivated Fields in Alberta, Canada. Geoderma 155:19-30 | en |
| dc.identifier.uri | http://hdl.handle.net/1993/4730 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | estrogen | en |
| dc.subject | immunoassay | en |
| dc.subject | sorption | en |
| dc.subject | mineralization | en |
| dc.title | Fate of estrogenic compounds in agricultural soils and development of an immunoassay for their environmental detection | en |
| dc.type | doctoral thesis | en_US |