Characterization of the fitness of adenoviral E1A exon 1 deletion mutants and the role of SUMOylation in DREF function

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dc.contributor.authorAkkerman, Nikolas
dc.contributor.examiningcommitteeMcKenna, Sean (Chemistry)en_US
dc.contributor.examiningcommitteeUyaguari-Diaz, Miguel (Microbiology)en_US
dc.contributor.supervisorPelka, Peter
dc.date.accessioned2022-07-05T14:09:58Z
dc.date.available2022-07-05T14:09:58Z
dc.date.copyright2022-07-04
dc.date.issued2022-07-04
dc.date.submitted2022-07-04T16:52:33Zen_US
dc.degree.disciplineMicrobiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractEarly region 1A (E1A) protein is the first protein expressed during the progression of adenoviral infection. E1A proteins push terminally differentiated cells back into the cell cycle through both altered cellular gene expression and activation of viral genes to start viral replication. E1A deletion mutant viruses have been used as reagents in a multitude of studies. My studies have characterized the viral growth, cytopathic effect (CPE), and viral genome replication of E1A exon 1 deletion mutants and revealed a mutant that both grows and replicates genomes at a higher rate than wildtype. The previous discovery of cellular transcription factor DREF being SUMOylated and this SUMOylation increasing globally during adenovirus infection warranted further study. My studies have shown that adenoviral growth is positively impacted by DREF SUMOylation and that expression of interferon stimulated genes (ISGs) and p53-related genes (PRGs) are affected by DREF SUMOylation in both healthy and adenoviral infected cells. This work allows for further characterization and specific use of E1A exon 1 deletion mutants and additional investigations into the role of DREF and its SUMOylation on the expression of anti-viral genes including ISGs and PRGs.en_US
dc.description.noteOctober 2022en_US
dc.identifier.urihttp://hdl.handle.net/1993/36603
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectCPEen_US
dc.subjectdeltion mutanten_US
dc.subjectDREFen_US
dc.subjectE1Aen_US
dc.subjectexon 1en_US
dc.subjectinterferonen_US
dc.subjectp53en_US
dc.subjectSUMOylationen_US
dc.subjectviral genome replicationen_US
dc.subjectviral growthen_US
dc.titleCharacterization of the fitness of adenoviral E1A exon 1 deletion mutants and the role of SUMOylation in DREF functionen_US
dc.typemaster thesisen_US
local.subject.manitobanoen_US
project.funder.identifierhttps://doi.org/10.13039/501100000038, https://doi.org/10.13039/501100000024, https://doi.org/10.13039/100010318en_US
project.funder.nameNatural Sciences and Engineering Research Council of Canada, Canadian Institutes of Health Research, University of Manitobaen_US
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