Influence of environmental factors on bovine mammary bacterial communities and mammary inflammation

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dc.contributor.authorFehr, Kelsey
dc.contributor.examiningcommitteeEmma, McGeough (Animal Science) Nazim, Cicek (Biosystems Engineering)en_US
dc.contributor.supervisorKhafipour, Ehsan (Animal Science) Plaizier, Jan C. (Animal Science)en_US
dc.date.accessioned2018-01-10T15:18:10Z
dc.date.available2018-01-10T15:18:10Z
dc.date.issued2017
dc.degree.disciplineAnimal Scienceen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractBovine milk and teat-apex bacterial communities are thought to contribute to mammary health. However, milk and teat-apex bacterial communities likely also differ in bacterial diversity and composition among farm-systems and between seasons, along with differing environmental bacterial communities. To find evidence supporting these notions, the bacterial-profile of milk, teat-apices, used-bedding and feces were assessed on three dairy farms using different bedding [Straw (ST), Sand (SA), and Recycled bedding material (RBM)], and also during summer and winter time-points for farm ST. Differences in between-sample bacterial diversity were identified among farms, and between both time-points for farm ST, for all sample-types (P(PERMANOVA)≤0.0002). Consistently among farms, a major source of milk microbiota was the teat-apex. Further, four OTUs on the teat-apex were associated with reduced mammary inflammation, and two within milk (P(FDR)<0.05). Overall, this research demonstrates farm and seasonal differences as substantial drivers in milk and teat-apex bacterial diversity and composition.en_US
dc.description.noteFebruary 2018en_US
dc.identifier.urihttp://hdl.handle.net/1993/32765
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectDairyen_US
dc.subjectUdderen_US
dc.subjectMammaryen_US
dc.subjectMicrobiomeen_US
dc.subjectEnvironmenten_US
dc.subjectMicrobiotaen_US
dc.subjectMastitisen_US
dc.titleInfluence of environmental factors on bovine mammary bacterial communities and mammary inflammationen_US
dc.typemaster thesisen_US
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Additional file 3. PerMANOVA main test and pairwise test results for weighted UniFrac distance metrics between samples of milk, teat-apex, fecal and bedding.
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Additional file 4. Bacterial profile details for phyla present in at least 25% of samples within a site.
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Additional file 5. Associations between phyla in each site, and metadata using boosted general linear models (MaAsLin results).
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