Enhanced biodenitrification is a technique for remediation of nutrient impacts that uses electron donor amendments to stimulate the microbial community present in natural groundwater aquifers. This study evaluated ethanol as a carbon source to decrease elevated nutrients in groundwater and soil microcosms under cold climate conditions. Initial microcosm nutrient concentrations of ammonia-N, nitrate-N, and nitrite-N were 2010 mg/L, 1690 mg/L, and 118 mg/L, respectively. Ethanol treatments showed that the nitrate-N and nitrite-N concentrations decreased to non-detectable limits in 56 days and ammonia-N decreased by 48% in 231 days. A second experiment examined the effect of vadose zone ethanol injections into nutrient impacted soil columns. The study indicated the ethanol amendments degraded 1.3, 13.9, and 27.2% of nitrate-N concentrations in the three soil columns. However, the vadose zone showed a decrease of 92.4 to 99.4% due to displacement and degradation. Overall biodenitrification shows promise as a cost-effective remedial solution.