Targeted disruption of the insulin-like growth factor binding protein-3 gene in mouse embryonic stem cells
| dc.contributor.author | Fu, Qiang | en_US |
| dc.date.accessioned | 2007-05-15T19:03:33Z | |
| dc.date.available | 2007-05-15T19:03:33Z | |
| dc.date.issued | 1998-05-01T00:00:00Z | en_US |
| dc.degree.discipline | Physiology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Insulin-like growth factors (IGFs) play important functions in cell proliferation, differentiation and metabolism. The biological actions of IGFs are mediated through binding to the specific receptor-IGF-I receptor. However, in the circulation IGFs are associated with their binding proteins: the insulin-like growth factor binding proteins (IGFBPs). Among the six IGFBPs, IGFBP-3 is the predominant binding protein for IGFs in circulation. IGFBP-3 is believed to play important regulatory roles in the biological actions of IGFs. Although many functions of IGFBP-3 have been described in the last decade, the results were not consistent. The physiological function of IGFBP-3 has not yet been defined. To study the physiological function of IGFBP-3, gene targeting technique was employed to generate a null IGFBP-3 mouse model. The mouse IGFBP-3 gene was obtained by screening a mouse genomic library using a full lengt human IGFBP-3 cDNA. The mouse IGFBP-3 gene restriction map was established by restriction analysis of the mouse IGFBP-3 gene by using different fragments of human IGFBP-3 cDNA as probes. (Abstract shortened by UMI.) | en_US |
| dc.format.extent | 4710639 bytes | |
| dc.format.extent | 184 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.format.mimetype | text/plain | |
| dc.identifier.uri | http://hdl.handle.net/1993/1140 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.title | Targeted disruption of the insulin-like growth factor binding protein-3 gene in mouse embryonic stem cells | en_US |
| dc.type | master thesis | en_US |