A microfluidic method for selecting chemotactic stem cells

Simple item page
dc.contributor.authorNatarajan, Kanmani
dc.contributor.examiningcommitteeHuebner, Erwin (Biological Sciences) Tian, Ganghong (Physiology and Pathophysiology)en_US
dc.contributor.supervisorLin, Francis (Physics and Astronomy)en_US
dc.date.accessioned2014-12-18T23:18:02Z
dc.date.available2014-12-18T23:18:02Z
dc.date.issued2014-12-18
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractStem cells hold great promise for treating various degenerative diseases. However, the outcomes of preclinical and clinical cell therapy studies are still not close to our expectation. The unsatisfactory outcomes of cell therapy are at least partially due to: 1) insufficient homing of implanted stem cells into target organs and 2) use of heterogeneous cell populations for cell therapy. Therefore, there is a need to develop effective guiding technique for stem cells to migrate to the target organs and to isolate effective stem cell populations. In this project, I developed a microfluidics-based method for selecting chemotactic adipose-derived stem cells (ASCs) to epidermal growth factor (EGF). This method integrates cell patterning, chemotaxis and cell extraction on a single microfluidic device. Post-extraction analysis confirmed the higher chemotactic ability of the extracted cells to EGF. The extracted chemotactic ASCs shows up-regulated surface expression of EGF receptor and its downstream signaling event upon EGF stimulation.en_US
dc.description.noteFebruary 2015en_US
dc.identifier.urihttp://hdl.handle.net/1993/30134
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectStem cellsen_US
dc.subjecthomingen_US
dc.subjectmicrofluidicsen_US
dc.subjectchemotactic ASCsen_US
dc.titleA microfluidic method for selecting chemotactic stem cellsen_US
dc.typemaster thesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Natarajan_Kanmani.pdf
Size:
2.19 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.25 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
FGS - Electronic Theses and Practica