Investigations of Single Cell Oils (SCOs): an analysis of growth and lipid biosynthesis in oleaginous microbes for biodiesel production
| dc.contributor.author | Sestric, Ryan | |
| dc.contributor.examiningcommittee | Cicek, Nazim (Biosystems Engineering) Court, Deborah (Microbiology) French, W. Todd (Chemical Engineering) Mississippi State University | en_US |
| dc.contributor.supervisor | Levin, David, B. (Biosystems Engineering) | en_US |
| dc.date.accessioned | 2016-06-03T18:07:28Z | |
| dc.date.available | 2016-06-03T18:07:28Z | |
| dc.date.issued | 2013-09 | en_US |
| dc.date.issued | 2014-07 | en_US |
| dc.degree.discipline | Biosystems Engineering | en_US |
| dc.degree.level | Doctor of Philosophy (Ph.D.) | en_US |
| dc.description.abstract | Biodiesel production from the triglycerides of Single Cell Organisms (SCO) has been researched for many years, and could supplement the transportation fuel market. Several improvements to the understanding of microbial physiology have led to increased lipid yields and reduction in costs of biodiesel production. Microalgae and oleaginous yeasts have been at the forefront of research for SCO production, and lipid biosynthesis is stimulated by nutrient limitation in both. The microalga Chlamydomonas reinhardtii BAFJ5, a mutant strain blocked for starch granule synthesis was grown heterotrophically and mixotrophically in both nutrient limited and rich media. The lipid production in heterotrophic growth was similar to mixotrophically grown cells. Following this, an extensive screen of known and uncharacterized oleaginous yeast assessed the lipid profiles and biomass concentrations of 69 strains. The strain Yarrowia lipolytica was chosen for further characterization, demonstrating its ability to metabolize biodiesel derived ‘waste’ glycerol and the effect it has on lipid synthesis. Pure and waste glycerol were both acceptable carbon substrates for biomass production, and no deleterious effects to the cell were seen. Finally, the proteome of Y. lipolytica was examined, which showed glycolysis, the tricarboxylic cycle, and lipid biosynthesis had similar regulation at the protein level under nitrogen-limited and nitrogen-sufficient conditions. | en_US |
| dc.description.note | October 2016 | en_US |
| dc.identifier.citation | Sitepu, Irnayuli R., et al. "Manipulation of culture conditions alters lipid content and fatty acid profiles of a wide variety of known and new oleaginous yeast species." Bioresource technology 144 (2013): 360-369. | en_US |
| dc.identifier.citation | Sestric, Ryan, et al. "Growth and neutral lipid synthesis by Yarrowia lipolytica on various carbon substrates under nutrient-sufficient and nutrient-limited conditions." Bioresource technology 164 (2014): 41-46. | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/31313 | |
| dc.language.iso | eng | en_US |
| dc.publisher | Elsevier | en_US |
| dc.publisher | Elsevier | en_US |
| dc.rights | open access | en_US |
| dc.subject | biofuels, yeast, microbiology, biotechnology, microalgae | en_US |
| dc.title | Investigations of Single Cell Oils (SCOs): an analysis of growth and lipid biosynthesis in oleaginous microbes for biodiesel production | en_US |
| dc.type | doctoral thesis | en_US |