Infectious complications associated with the use of commercial bioengineered collagen-GAG matrices are of concern due to the high incidence of infection, difficulty of accurately discerning the presence of infection, and high cost and delayed wound healing when it becomes necessary to excise infected areas. We modified the collagen layer of a commercial bioengineered collagen- GAG matrix with a ciprofloxacin-based pro-drug “Pro-Cip” anchored to the matrix by a polydopamine layer in a simple one-pot chemistry. An ad-layer coating strategy enriched the surface with Pro-Cip, and various boosters for lipase activity were added to further enhance the antibacterial potency of the coating. The coated bioengineered collagen-GAG matrix exhibited potent antibacterial activity against MRSA and P. aeruginosa, achieving a complete bacterial eradication (no detectable CFU with a detection limit of 33 CFU/mL) within 18 hours at low initial inoculum (~10^4 CFU/mL). When challenged with a higher bacterial burden (10^8 CFU/mL), the coated bioengineered collagen-GAG matrix demonstrated robust antimicrobial efficacy, resulting in 100% (8.0) log reduction in MRSA and P. aeruginosa within 5h contact. Zone of inhibition testing yielded clear zones of up to 25 mm diameter, which highlights the ability of the antibiotic to diffuse into the infected wound after bacteria trigger its release from the coated surface. Furthermore, the surface-modified bioengineered collagen-GAG matrix retained excellent cell- adhesion and proliferation, and cell viability remained at 95% after exposure to membrane elutions compared to the unmodified bioengineered collagen-GAG matrix. The self-disinfecting properties of the modified bioengineered collagen-GAG matrix are anticipated to significantly enhance patient outcomes by mitigating the substantial risk of infections, a critical factor in the management of severe wounds such as burns.