Evaluation of Influenza-Specific Humoral Response by Microbead Array Analysis

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dc.contributor.authorKeynan, Yoav
dc.contributor.authorBodnarchuk, Tavis
dc.contributor.authorWayne, Stephen
dc.contributor.authorLi, Yan
dc.contributor.authorFowke, Keith R.
dc.date.accessioned2016-06-07T21:02:24Z
dc.date.available2016-06-07T21:02:24Z
dc.date.issued2011-1-1
dc.date.updated2016-06-07T06:55:48Z
dc.description.abstractRATIONALE: Quantitation and determination of antigen specificity of systemic and mucosal immune responses to influenza vaccination is beneficial for future vaccine development. Previous methods to acquire this information were costly, time consuming and sample exhaustive. The benefits of suspension microbead array (MBA) analysis are numerous. The multiplex capabilities of the system conserve time, money and sample, while generating statistically powerful data.OBJECTIVE: To demonstrate the use of the assay by comparing the humoral influenza-specific responses of two cohorts from two countries that differed in circulating influenza strains and rates of influenza vaccination.METHODS: Influenza hemagglutinin (HA) from different strains were coated on microbeads and incubated with serum samples to capture immunoglobulin (Ig) A1 and IgG1 host antibodies.RESULTS: Statistically significant differences in IgA1 and IgG1 exist between the serum samples from Winnipeg (Manitoba) donors and those from Kenyan (Africa) donors. Data were compared using Mann-Whitney nonparametric tests. The Winnipeg donors had higher mean fluorescence intensity values, with significant P values for anti-HA IgA1 to A/Wyoming/3/2003 (P=0.044), A/Vietnam/1203/2004 (P=0.0179), A/New Caledonia/20/99 (Pud_less_than0.0001) and B/Tokyo/53/99 (P=0.0002). No differences were seen between the groups in their response to B/Jilin/20/2003. The Winnipeg donors had higher mean fluorescence intensity values, with significant P values for anti-HA IgG1 to A/Wyoming/3/2003 (P=0.0135), B/Tokyo/53/99 (P=0.006) and B/Jilin20/2003 (P=0.026).CONCLUSION: Influenza-specific IgA1 and IgG1 antibodies were successfully detected using MBA technology. A significant difference in antibody response was observed between Winnipeg and Kenyan donor serums. MBA analysis is a relatively quick and cost-effective method for serum antibody analysis. The potential to simultaneously assay small sample volumes for a multitude of antigens makes this method invaluable for future vaccine response monitoring.
dc.description.versionPeer Reviewed
dc.identifier.citationYoav Keynan, Tavis Bodnarchuk, Stephen Wayne, Yan Li, and Keith R. Fowke, “Evaluation of Influenza-Specific Humoral Response by Microbead Array Analysis,” Canadian Journal of Infectious Diseases and Medical Microbiology, vol. 22, no. 1, pp. 25-29, 2011. doi:10.1155/2011/202516
dc.identifier.urihttp://dx.doi.org/10.1155/2011/202516
dc.identifier.urihttp://hdl.handle.net/1993/31368
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderCopyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleEvaluation of Influenza-Specific Humoral Response by Microbead Array Analysis
dc.typeJournal Article
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