Early Growth Response 2 (Egr2) Induction by Neuronal Activity-dependent Nuclear Factor Kappa B (NF-κB) Activation
| dc.contributor.author | Nafez, Solmaz | |
| dc.contributor.examiningcommittee | Smyth, Donald (Pharmacology and Therapeutics) Eisenstat, David (Biochemistry and Medical Genetics) Glazner, Gordon (Pharmacology and Therapeutics) | en |
| dc.contributor.supervisor | Albensi, Benedict (Pharmacology and Therapeutics) | en |
| dc.date.accessioned | 2010-09-13T16:37:24Z | |
| dc.date.available | 2010-09-13T16:37:24Z | |
| dc.date.issued | 2010-09-13T16:37:24Z | |
| dc.degree.discipline | Pharmacology and Therapeutics | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Nuclear factor kappa B (NF-κB) mediated signalling is complex and plays a critical role in many biological processes. Investigators have reported that NF-κB is activated during the induction of long term potentiation (LTP), a proposed mechanism for memory encoding, and may be a requirement for synaptic plasticity and memory. In this study, mRNA extracted from hippocampal slices of NF-kB p50 knockout mice and its littermate before and after induction of LTP was analyzed using DNA microarray analysis (Affy-metrix GeneChip® Mouse Genome 430 2.0) to explore candidate target genes of NF-kB in LTP. The early growth response 2 (Egr-2) was identified as one putative NF-kB target gene. Egr-2 mRNA and protein analysis of primary cortical neurons and HeLa cells chemically stimulated with Tumor Necrosis Factor α (TNFα) to activate the NF-kB sig-nalling pathway confirmed the microarray results. In addition, examination of the Egr-2 promoter sequence for NF-kB binding sites using chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assays (EMSA) confirmed promoter occupancy and specificity of binding in vivo, respectively. These data suggest that Egr-2 expression level is controlled by direct transcriptional activity of the NF-kB transcription factor. | en |
| dc.description.note | October 2010 | en |
| dc.format.extent | 1608512 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/1993/4191 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | neuroscience | en |
| dc.subject | molecular biology | en |
| dc.subject | memory function | en |
| dc.subject | Alzheimer's disease | en |
| dc.title | Early Growth Response 2 (Egr2) Induction by Neuronal Activity-dependent Nuclear Factor Kappa B (NF-κB) Activation | en |
| dc.type | master thesis | en_US |