Role of liver fatty acid binding protein in fatty liver cell culture model
| dc.contributor.author | Chen, Yufei | |
| dc.contributor.examiningcommittee | Gong, Yuewen (Pharmacy) Minuk, Gerald Y (Pharmacology and Therapeutics) | en_US |
| dc.contributor.supervisor | Burczynski, Frank J (Pharmacy) | en_US |
| dc.date.accessioned | 2012-04-05T15:22:42Z | |
| dc.date.available | 2012-04-05T15:22:42Z | |
| dc.date.issued | 2012-04-05 | |
| dc.degree.discipline | Pharmacy | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Liver fatty acid binding protein has been reported to possess antioxidant properties in the liver. The aim of this study was to investigate the effect of this protein in a nonalcoholic fatty liver disease (NAFLD) cell culture model. Rat hepatoma cells were treated with an oleate:palmitate (2:1) mixture for either 1 and 2 days, or further treated with 500 µM clofibrate to induce L-FABP expression. Intracellular lipid accumulation was quantitated by Nile Red. Lipotoxicity was determined using the WST-1 assay. Dichlorofluorescein (DCF) was utilized to assess intracellular reactive oxidative species (ROS) level. Measurement of lipotoxicity showed statistical decreases in cell viability as lipid concentrations increased in a dose-dependent manner. NAFLD cell cultures showed characteristic cellular damage from increased ROS levels in fatty acid treated cells. All groups treated with clofibrate showed statistically increased intracellular L-FABP levels and reduced ROS levels. The results lead to the conclusion that clofibrate induces L-FABP expression and in this manner suppresses hepatocellular ROS generation. | en_US |
| dc.description.note | May 2012 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/5260 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | L-FABP | en_US |
| dc.subject | NAFLD | en_US |
| dc.subject | ROS | en_US |
| dc.subject | antioxidant therapy | en_US |
| dc.subject | oxidative stress | en_US |
| dc.title | Role of liver fatty acid binding protein in fatty liver cell culture model | en_US |
| dc.type | master thesis | en_US |