Wettability of nanofibrous membrane regulating stem cell differentiation

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dc.contributor.authorGao, Haiyun
dc.contributor.examiningcommitteeKing, Martin W.(Textile Sciences) Chen, Ying (Biosystems Engineering)en_US
dc.contributor.supervisorZhong, Wen (Textile Science) Xing, Malcolm (Mechanical and Manufacturing Engineering)en_US
dc.date.accessioned2013-01-08T21:59:24Z
dc.date.available2013-01-08T21:59:24Z
dc.date.issued2013-01-08
dc.degree.disciplineTextile Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractIn this work, I investigated the influence of different surfaces on stem cell proliferation and osteogenetic differentiation. Surface properties of biomaterials are important factors that influence cell fate such as cell adhesion, viability, proliferation and differentiation. Herein, mesenchymal stem cells (MSCs) were cultured on composite electrospun nanofibrous membranes with varied surface wettability for designed periods and cell morphologies, proliferation and viability were characterized via analysis methods such as Infrared attenuated total reflectance Spectroscopy (IR-ATR), scanning electron microscopy (SEM) and MTT cell proliferation assay. The expression of genes associated with osteogenesis, including bone sialoprotein (BSP), alkaline phosphatase (ALP), osteopontin (OPN) and osteocalcin (OCN) were measured by real-time RT-PCR on different time points. Through western blot analysis, ERK1/2 pathway was found to be responsible for the differentiation of MSCs on nanofibrous membranes with different wettability.en_US
dc.description.noteFebruary 2013en_US
dc.identifier.urihttp://hdl.handle.net/1993/14429
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectstem cellen_US
dc.subjectnanofibrous membraneen_US
dc.titleWettability of nanofibrous membrane regulating stem cell differentiationen_US
dc.typemaster thesisen_US
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