Toll-like Interleukin -1 Receptor Regulator (TILRR) Protein, a Major Modulator of Inflammation, is Expressed in Normal Human and Macaque Tissues and PBMCs

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dc.contributor.authorKashem, Mohammad Abul
dc.contributor.authorLuo, Ma
dc.date.accessioned2022-05-11T22:22:57Z
dc.date.available2022-05-11T22:22:57Z
dc.date.issued2022-05-11
dc.date.submitted2022-05-11T18:24:13Zen_US
dc.description.abstractPurpose: TILRR is a modulator of genes in the NF-κB inflammation pathway. It regulates inflammation-responsive genes, the secretion of inflammatory mediators, and the migration of immune cells. Because inflammation drives the pathogenesis of many infectious and inflammatory diseases, it is important to know the expression of TILRR protein in tissues and cells. This study examined TILRR protein expression in healthy adult human and macaques’ tissues and PBMCs (peripheral blood mononuclear cells). Methods and Results: Tissues (trachea, lungs, stomach, small intestine [ileum], cecum, colon, rectum, vagina, cervix, uterus, and penis) and PBMCs from humans and macaques were lysed in RIPA (radioimmunoprecipitation assay) lysis buffer. The TILRR protein was examined by fluorescent Western blot analysis. The relative fluorescence units (rfu) of TILRR protein expression were quantified by Image Studio software (LI-COR). The results showed that adult healthy female (n=1) rectal and cervicovaginal tissues expressed a higher level of TILRR protein than the other tissues (trachea, lungs, stomach, small intestine [ileum], cecum, colon, uterus, and penis) examined. Like humans, the lungs, colon, and rectal tissues of healthy adult female cynomolgus monkeys (Macaca fascicularis) (n=2) expressed the TILRR protein. In addition, PBMCs of healthy adult women (n=4), adult female cynomolgus monkeys (Macaca fascicularis) (n=4), and adult male and female rhesus monkeys (Macaca mulatta) (n=4) showed a similar expression level of TILRR protein (p= 0.2858). TILRR protein was not detected in most of the human cell lines examined, except in Jurkat cells. Conclusion: Our study for the first time showed that TILRR protein is expressed in healthy adult human and monkey tissues and PBMCs. The TILRR protein in these tissues and PBMCs may play a role in the inflammatory response of these tissues and cells in response to infectious pathogens.en_US
dc.identifier.doihttps://doi.org/10.2147/JIR.S357866
dc.identifier.urihttp://hdl.handle.net/1993/36493
dc.language.isoengen_US
dc.publisherDove Pressen_US
dc.rightsopen accessen_US
dc.subjecttissuesen_US
dc.subjecthumanen_US
dc.subjectrhesus monkeyen_US
dc.subjectMacaca mulattaen_US
dc.subjectcynomolgus monkeyen_US
dc.subjectMacaca fascicularisen_US
dc.subjectinflammationen_US
dc.titleToll-like Interleukin -1 Receptor Regulator (TILRR) Protein, a Major Modulator of Inflammation, is Expressed in Normal Human and Macaque Tissues and PBMCsen_US
dc.typeresearch articleen_US
local.author.affiliationRady Faculty of Health Sciences::Max Rady College of Medicine::Department of Medical Microbiology and Infectious Diseasesen_US
oaire.awardTitleCHVI Vaccine Discovery and Social Researchen_US
oaire.citation.endPage2937en_US
oaire.citation.startPage2925en_US
oaire.citation.titleJournal of Inflammation Researchen_US
oaire.citation.volume15en_US
project.funder.identifierhttps://doi.org/10.13039/501100000024en_US
project.funder.nameCanadian Institutes of Health Researchen_US
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