Mitochondrial BNIP3 upregulation precedes endonuclease G translocation in hippocampal neuronal death following oxygen-glucose deprivation

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dc.contributor.authorZhao, Shen-Ting
dc.contributor.authorChen, Ming
dc.contributor.authorLi, Shu-Ji
dc.contributor.authorZhang, Ming-Hai
dc.contributor.authorLi, Bo-Xing
dc.contributor.authorDas, Manas
dc.contributor.authorBean, Jonathan C
dc.contributor.authorKong, Ji-Ming
dc.contributor.authorZhu, Xin-Hong
dc.contributor.authorGao, Tian-Ming
dc.date.accessioned2013-10-01T19:13:12Z
dc.date.available2013-10-01T19:13:12Z
dc.date.issued2009-09-08
dc.date.updated2013-10-01T19:13:13Z
dc.description.abstractAbstract Background Caspase-independent apoptotic pathways are suggested as a mechanism for the delayed neuronal death following ischemic insult. However, the underlying signalling mechanisms are largely unknown. Recent studies imply the involvement of several mitochondrial proteins, including endonuclease G (EndoG) and Bcl-2/adenovirus E1B 19 kDa-interacting protein (BNIP3), in the pathway of non-neuronal cells. Results In this report, using western blot analysis and immunocytochemistry, we found that EndoG upregulates and translocates from mitochondria to nucleus in a time-dependent manner in cultured hippocampal neurons following oxygen-glucose deprivation (OGD). Moreover, the translocation of EndoG occurs hours before the observable nuclear pyknosis. Importantly, the mitochondrial upregulation of BNIP3 precedes the translocation of EndoG. Forced expression of BNIP3 increases the nuclear translocation of EndoG and neuronal death while knockdown of BNIP3 decreases the OGD-induced nuclear translocation of EndoG and neuronal death. Conclusion These results suggest that BNIP3 and EndoG play important roles in hippocampal neuronal apoptosis following ischemia, and mitochondrial BNIP3 is a signal protein upstream of EndoG that can induce neuronal death.
dc.description.versionPeer Reviewed
dc.identifier.citationBMC Neuroscience. 2009 Sep 08;10(1):113
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2202-10-113
dc.identifier.urihttp://hdl.handle.net/1993/22214
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderShen-Ting Zhao et al.; licensee BioMed Central Ltd.
dc.titleMitochondrial BNIP3 upregulation precedes endonuclease G translocation in hippocampal neuronal death following oxygen-glucose deprivation
dc.typeJournal Article
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