Paracrine signalling between keratinocytes and SVF cells results in a new secreted cytokine profile during wound closure

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dc.contributor.authorBalko, Stefan
dc.contributor.authorKerr, Evan
dc.contributor.authorBuchel, Edward
dc.contributor.authorLogsetty, Sarvesh
dc.contributor.authorRaouf, Afshin
dc.date.accessioned2023-11-07T18:32:00Z
dc.date.available2023-11-07T18:32:00Z
dc.date.issued2023-09-19
dc.date.updated2023-11-03T09:17:58Z
dc.description.abstractStromal vascular fraction (SVF) cells, and the adipose-derived mesenchymal stem cells they contain, have shown enhanced wound healing in vitro and in vivo, yet their clinical application has been limited. In this regard, understanding the mechanisms that govern SVF-enhanced wound healing would improve their application in the clinic. Here, we show that the SVF cells and keratinocytes engage in a paracrine crosstalk during wound closure, which results in a new cytokine profile that is distinct from the cytokines regularly secreted by either cell type on their own. We identify 11 cytokines, 5 of which are not regularly secreted by the SVF cells, whose expressions are significantly increased during wound closure by the keratinocytes. This new cytokine profile could be used to accelerate wound closure and initiate re-epithelialization without the need to obtain the SVF cells from the patient.
dc.identifier.citationStem Cell Research & Therapy. 2023 Sep 19;14(1):258
dc.identifier.doi10.1186/s13287-023-03488-0
dc.identifier.urihttp://hdl.handle.net/1993/37769
dc.language.isoeng
dc.language.rfc3066en
dc.publisherBMC
dc.rightsopen accessen_US
dc.rights.holderBioMed Central Ltd., part of Springer Nature
dc.subjectWound healing
dc.subjectScratch assay
dc.subjectCell migration
dc.subjectKeratinocytes
dc.subjectFibroblasts
dc.subjectCytokines
dc.titleParacrine signalling between keratinocytes and SVF cells results in a new secreted cytokine profile during wound closure
dc.typeJournal Article
local.author.affiliationRady Faculty of Health Sciences::Max Rady College of Medicine::Department of Immunology
oaire.citation.issue258
oaire.citation.titleStem Cell Research & Therapy
oaire.citation.volume14
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