CD8aa dimer is a receptor for Nipah virus on porcine lymphocytes

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dc.contributor.authorTailor, Nikesh
dc.contributor.examiningcommitteeDrebot, Michael (Medical Microbiology and Infectious Diseases) Yang, Xi (Immunology) Nfon, Charles (Animal Science)en_US
dc.contributor.supervisorWeingartl, Hana (Medical Microbiology and Infectious Diseases)en_US
dc.date.accessioned2019-09-05T15:45:20Z
dc.date.available2019-09-05T15:45:20Z
dc.date.issued2019-07-11en_US
dc.date.submitted2019-07-11T21:15:03Zen
dc.degree.disciplineMedical Microbiology and Infectious Diseasesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractNipah virus (NiV) and Hendra Virus (HeV) (Paramyxoviridae; Henipavirus) cause severe often fatal illness in humans, but can also infect swine. The presented work compared permissibility of porcine peripheral blood mononuclear cells to NiV and to HeV. While majority of cell populations did not support replication of either HeV or NiV, monocytes were permissive to both viruses. CD8+ subpopulations of T and NK cells were permissive only to NiV, even though they did not express ephrin B2 (receptor for HeV and NiV) on the surface. CHO-K1 cells transfected with porcine CD8α became permissive to NiV. Antibody against CD8α was able to block NiV replication in CD8+ cells; competition assays between HeV or NiV soluble virus attachment protein (sG) suggested that NiV can bind to CD8αα expressing porcine cells while HeV cannot. CD8αβ cells were not permissive to NiV or HeV. The results indicate that porcine CD8α dimer is a receptor for Nipah virus, but not for HeV on porcine lymphocytes. This work has implications in vaccine design. Development of a veterinary vaccine against NiV which elicits cell mediated immune respose is needed.en_US
dc.description.noteOctober 2019en_US
dc.identifier.urihttp://hdl.handle.net/1993/34164
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectNipah Virusen_US
dc.subjectHendra Virusen_US
dc.subjectMedical microbiologyen_US
dc.subjectMicrobiologyen_US
dc.subjectVirologyen_US
dc.subjectImmunologyen_US
dc.titleCD8aa dimer is a receptor for Nipah virus on porcine lymphocytesen_US
dc.typemaster thesisen_US
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