Canadian Public Health Laboratory Network Laboratory Guidelines for the Use of Direct Tests to Detect Syphilis in Canada

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dc.contributor.authorTsang, Raymond SW
dc.contributor.authorMorshed, Muhammad
dc.contributor.authorChernesky, Max A
dc.contributor.authorJayaraman, Gayatri C
dc.contributor.authorKadkhoda, Kamran
dc.date.accessioned2016-06-07T17:14:28Z
dc.date.available2016-06-07T17:14:28Z
dc.date.issued2015-1-1
dc.date.updated2016-06-07T06:51:54Z
dc.description.abstractTreponema pallidum subsp. pallidum and/or its nucleic acid can be detected by various methods such as microscopy, rabbit infectivity test or polymerase chain reaction (PCR) tests. The rabbit infectivity test for T. pallidum, although very sensitive, has been discontinued from most laboratories due to ethical issues related to the need for animal inoculation with live T. pallidum, the technically demanding procedure and long turnaround time for results, thus making it impractical for routine diagnostic use. Dark-field and phase-contrast microscopy are still useful at clinic- or hospital-based laboratories for near-bedside detection of T. pallidum in genital, skin or mucous lesions although their availability is decreasing. The lack of reliable and specific anti-T. pallidum antibodies and its inferior sensitivity to PCR may explain why the direct fluorescent antibody test for T. pallidum is not widely available for clinical use. Immunohistochemical staining for T. pallidum also depends on the availability of specific antibodies, and the method is only applicable for histopathological examination of biopsy and autopsy specimens necessitating an invasive specimen collection approach. With recent advances in molecular diagnostics, PCR is considered to be the most reliable, versatile and practical for laboratories to implement. In addition to being an objective and sensitive test for direct detection of Treponema pallidum subsp. pallidum DNA in skin and mucous membrane lesions, the resulting PCR amplicons from selected gene targets can be further characterized for antimicrobial (macrolide) susceptibility testing, strain typing and identification of T. pallidum subspecies.
dc.description.versionPeer Reviewed
dc.identifier.citationRaymond SW Tsang, Muhammad Morshed, Max A Chernesky, Gayatri C Jayaraman, and Kamran Kadkhoda, “Canadian Public Health Laboratory Network Laboratory Guidelines for the Use of Direct Tests to Detect Syphilis in Canada,” Canadian Journal of Infectious Diseases and Medical Microbiology, vol. 26, no. Supplement A, pp. 13A-17A, 2015. doi:10.1155/2015/685603
dc.identifier.urihttp://dx.doi.org/10.1155/2015/685603
dc.identifier.urihttp://hdl.handle.net/1993/31324
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderCopyright © 2015 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.titleCanadian Public Health Laboratory Network Laboratory Guidelines for the Use of Direct Tests to Detect Syphilis in Canada
dc.typeJournal Article
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