Distinct cervical tissue-adherent and luminal microbiome communities correlate with mucosal host gene expression and protein levels in Kenyan sex workers

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dc.contributor.authorEdfeldt, Gabriella
dc.contributor.authorKaldhusdal, Vilde
dc.contributor.authorCzarnewski, Paulo
dc.contributor.authorBradley, Frideborg
dc.contributor.authorBergström, Sofia
dc.contributor.authorLajoie, Julie
dc.contributor.authorXu, Jiawu
dc.contributor.authorManberg, Anna
dc.contributor.authorKimani, Joshua
dc.contributor.authorOYUGI, JULIUS
dc.contributor.authorNilsson, Peter
dc.contributor.authorTjernlund, Annelie
dc.contributor.authorFowke, Keith
dc.contributor.authorKwon, Douglas S.
dc.contributor.authorBroliden, Kristina
dc.date.accessioned2023-05-01T15:38:51Z
dc.date.available2023-05-01T15:38:51Z
dc.date.issued2023-03-31
dc.date.updated2023-04-04T17:42:45Z
dc.description.abstractAbstract Background The majority of studies characterizing female genital tract microbiota have focused on luminal organisms, while the presence and impact of tissue-adherent ectocervical microbiota remain incompletely understood. Studies of luminal and tissue-associated bacteria in the gastrointestinal tract suggest that these communities may have distinct roles in health and disease. Here, we performed a multi-omics characterization of paired luminal and tissue samples collected from a cohort of Kenyan female sex workers. Results We identified a tissue-adherent bacterial microbiome, with a higher alpha diversity than the luminal microbiome, in which dominant genera overall included Gardnerella and Lactobacillus, followed by Prevotella, Atopobium, and Sneathia. About half of the L. iners-dominated luminal samples had a corresponding Gardnerella-dominated tissue microbiome. Broadly, the tissue-adherent microbiome was associated with fewer differentially expressed host genes than the luminal microbiome. Gene set enrichment analysis revealed that L. crispatus-dominated tissue-adherent communities were associated with protein translation and antimicrobial activity, whereas a highly diverse microbial community was associated with epithelial remodeling and pro-inflammatory pathways. Tissue-adherent communities dominated by L. iners and Gardnerella were associated with lower host transcriptional activity. Tissue-adherent microbiomes dominated by Lactobacillus and Gardnerella correlated with host protein profiles associated with epithelial barrier stability, although with a more pro-inflammatory profile for the Gardnerella-dominated microbiome group. Tissue samples with a highly diverse composition had a protein profile representing cell proliferation and pro-inflammatory activity. Conclusion We identified ectocervical tissue-adherent bacterial communities in all study participants of a female sex worker cohort. These communities were distinct from cervicovaginal luminal microbiota in a significant proportion of individuals. We further revealed that bacterial communities at both sites correlated with distinct host gene expression and protein levels. The tissue-adherent bacterial community could possibly act as a reservoir that seed the lumen with less optimal, non-Lactobacillus, bacteria. Video Abstracten_US
dc.identifier.citationMicrobiome. 2023 Mar 31;11(1):67
dc.identifier.citationMicrobiome. 2023 Mar 31;11(1):67
dc.identifier.urihttps://doi.org/10.1186/s40168-023-01502-4
dc.identifier.urihttp://hdl.handle.net/1993/37316
dc.language.isoengen_US
dc.language.rfc3066en
dc.publisherBioMed Central (BMC)en_US
dc.rightsopen accessen_US
dc.rights.holderThe Author(s)
dc.titleDistinct cervical tissue-adherent and luminal microbiome communities correlate with mucosal host gene expression and protein levels in Kenyan sex workersen_US
dc.typejournal articleen_US
local.author.affiliationRady Faculty of Health Sciences::Max Rady College of Medicine::Department of Medical Microbiology and Infectious Diseasesen_US
oaire.citation.issue1en_US
oaire.citation.startPage67en_US
oaire.citation.titleMicrobiomeen_US
oaire.citation.volume11en_US
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