Development of linkage map of Brassica juncea using molecular markers and detection of quantitative trait loci for oil content, seed protein and fatty acids

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dc.contributor.authorWatts, Roger
dc.contributor.examiningcommitteeLi, Genyi (Plant Science) Schroeder, Dana (Biological Sciences)en_US
dc.contributor.supervisorTahir, Muhammad (Plant Science)en_US
dc.date.accessioned2013-01-28T17:36:45Z
dc.date.available2013-01-28T17:36:45Z
dc.date.issued2013-01-28
dc.degree.disciplinePlant Scienceen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractA genetic linkage map of mustard (Brassica juncea) was developed using two double haploid populations produced from crosses between a low erucic cultivar “ZEM1” and two moderate erucic acid lines “Vniimk351” and “Vniimk405” with the use of SSR and SRAP markers. The linkage map of the ZEM1xVniimk351 population included 13 linkage groups with an overall length of 791 cM with an average marker interval of 5.7 cM. The linkage map of the ZEM1xVniimk405 population also contained 13 linkage groups with a distance of 623 cM and an average marker interval of 4.6 cM. Using the linkage maps for the two populations, QTLs were detected for seed oil, protein and fatty acids. QTL analysis for fatty acids indentified QTLs on LG1, 7 and 12 for the ZEM1xVniimk351 population and LG1, 3 and 4 for the ZEM1xVniimk405 population. Analysis for the seed oil and protein content in the ZEM1xVniimk351 population identified 2 QTLs on LG1 and LG4 and 1 QTL on LG1 respectively. The QTL analysis ZEM1xVniimk405 of oil and protein content identified 1 QTL for oil and protein on LG1. The variation of fatty acids was shown to be the result of monogenic inheritance of the FAE1 gene in both populations.en_US
dc.description.noteMay 2013en_US
dc.identifier.urihttp://hdl.handle.net/1993/15726
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectQTLen_US
dc.subjectJunceaen_US
dc.subjectSSRen_US
dc.subjectBrassicaen_US
dc.subjectSRAPen_US
dc.subjectLinkageen_US
dc.subjectMapen_US
dc.titleDevelopment of linkage map of Brassica juncea using molecular markers and detection of quantitative trait loci for oil content, seed protein and fatty acidsen_US
dc.typemaster thesisen_US
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