Impact of asthma, environmental exposures and ethnicity on functional responsiveness to Toll-like receptor (TLR) stimulation in children

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dc.contributor.authorLissitsyn, Yuriy V
dc.contributor.examiningcommitteeBecker, Allan (Pediatrics and Child Health) Marshall, Aaron (Immunology)Yang, Xi (Medical Microbiology)en
dc.contributor.supervisorHayGlass, Kent (Immunology)en
dc.date.accessioned2007-08-31T19:26:09Z
dc.date.available2007-08-31T19:26:09Z
dc.date.issued2007-08-31T19:26:09Z
dc.degree.disciplineImmunologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractTLRs play a key role in initiating innate immunity and in regulating the nature of the adaptive immune response. We hypothesized that functional responsiveness to TLR stimulation differs in clinically; environmentally; ethnically distinct pediatric populations. PBMC obtained from 272 children were stimulated with a panel of TLR ligands. Levels of pro- and anti-inflammatory, Th1-, Th2-associated cytokines were quantified by ELISA. We demonstrate that use of threshold concentrations of TLR4 and TLR2 ligands reveal striking differences in cytokine responses between asthmatic and non-atopic children. Specifically, non-atopic controls produce higher levels of pro-inflammatory cytokines, whereas asthmatics exhibit increased anti-inflammatory IL-10 responses. Asthmatic children exposed to environmental tobacco smoke (ETS) demonstrated elevated levels of chemokines relative to non-ETS exposed asthmatics and controls. First Nation children favor anti-inflammatory IL-10 responses, whereas Caucasian population respond to TLR activation by production of more robust pro-inflammatory and Th1 biased cytokine and chemokine responses.en
dc.description.noteOctober 2007en
dc.format.extent1426446 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1993/2765
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectasthmaen
dc.subjecttoll-like receptoren
dc.titleImpact of asthma, environmental exposures and ethnicity on functional responsiveness to Toll-like receptor (TLR) stimulation in childrenen
dc.typemaster thesisen_US
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