Regulation of podocalyxin expression in the kidney of streptozotocin-induced diabetic rats with Chinese herbs (Yishen capsule)

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dc.contributor.authorFang, Jingai
dc.contributor.authorWei, Hongkun
dc.contributor.authorSun, Yanyan
dc.contributor.authorZhang, Xiaodong
dc.contributor.authorLiu, Wenyuan
dc.contributor.authorChang, Qintao
dc.contributor.authorWang, Ruihua
dc.contributor.authorGong, Yuewen
dc.date.accessioned2013-04-26T11:06:52Z
dc.date.available2013-04-26T11:06:52Z
dc.date.issued2013-04-05
dc.date.updated2013-04-26T11:07:14Z
dc.description.abstractAbstract Background Diabetic nephropathy is an emergent issue in China with increase in patients with type II diabetes. There are several successful Chinese herbal products for the treatment of patients with diabetic nephropathy in China. However, the mechanisms mediating the biological activity of these products are still unclear. Podocalyxin is a sialoprotein critical to maintaining integrity of filtration function of glomerulus. Methods By employing streptozotocin-induced diabetic rats and a Chinese herb formulation (Yishen capsule), we examined the regulation of podocalyxin expression in the kidney by Yishen capsule through immunofluorescent staining and reverse transcriptase polymerase chain reaction. Results After injection of STZ, there were significant increase in both blood glucose and urinary protein. Serum creatinine and BUN were also increased in rats with injection of STZ. Moreover, expression of podocalyxin in the glomerulus was gradually reduced after injection of STZ. There was also a loss of podocyte foot processes in the glomerular basement membrane. However, Yishen capsule or benazepril was able to restore the expression of podocalyxin and podocyte foot processes in the kidney. Although Yishen capsule could reduce urinary protein level, it has little effect on blood glucose level in the rats injected with STZ. Conclusions Yishen capsule could attenuate the loss of podocalyxin in the glomerulus of rats injected with STZ.
dc.description.versionPeer Reviewed
dc.identifier.citationBMC Complementary and Alternative Medicine. 2013 Apr 05;13(1):76
dc.identifier.doihttp://dx.doi.org/10.1186/1472-6882-13-76
dc.identifier.urihttp://hdl.handle.net/1993/19685
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderJingai Fang et al.; licensee BioMed Central Ltd.
dc.titleRegulation of podocalyxin expression in the kidney of streptozotocin-induced diabetic rats with Chinese herbs (Yishen capsule)
dc.typeJournal Article
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