Prostate cancer circulating tumor cells: automated and manual enumeration after isolation via size-based filtration of pre-treatment patient samples.
| dc.contributor.author | Alsaadi, Hazem | |
| dc.contributor.examiningcommittee | Klonisch, Thomas (Human Anatomy and Cell Science) Drachenberg, Darrel (Surgery-Urology) | en_US |
| dc.contributor.supervisor | Mai, Sabine (Human Anatomy and Cell Science) | en_US |
| dc.date.accessioned | 2016-10-05T16:14:46Z | |
| dc.date.available | 2016-10-05T16:14:46Z | |
| dc.date.issued | 2016 | |
| dc.degree.discipline | Human Anatomy and Cell Science | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | CTCs have emerged as a potential source of clinical significance. But with numerous isolating systems currently available, the numbers of captured CTCs vary widely. At this point, CellSearch remains the only FDA-approved system with clinical significance whereby the results could be used to monitor patients with metastatic colon, breast, or prostate cancer. However, its inability to isolate CTCs from non-high risk prostate cancer patients or CTCs that are EpCAM-negative has led to criticism. In this study, we have shown that size-based filtration successfully isolates CTCs from patients with localized and metastatic prostate cancer. We have also shown that CTCs can be successfully isolated from low and intermediate risk groups. Additionally, clusters of CTCs were preserved and isolated in all localized risk groups and metastatic patients. Furthermore, we enumerated the isolated CTCs using automated and manual methods in low risk, intermediate risk, high risk, and metastatic prostate cancer. The automated and manual counts were comparable. Moreover, the amounts of clusters and the size of clusters correlated with the status and stage of prostate cancer. | en_US |
| dc.description.note | October 2016 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/31878 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Circulating Tumor Cells, Prostate Cancer, Filtration, ScreenCell, Androgen Receptor, Enumeration | en_US |
| dc.title | Prostate cancer circulating tumor cells: automated and manual enumeration after isolation via size-based filtration of pre-treatment patient samples. | en_US |
| dc.type | master thesis | en_US |