NeuroImmune modulation of multiple sclerosis via the dorsal root ganglia
| dc.contributor.author | Melanson, Maria | |
| dc.contributor.examiningcommittee | Kreillaars, Dean (Medical Rehabilitation) Shay, Barbara (Medical Rehabilitation) | en |
| dc.contributor.supervisor | Namaka, Mike (Pharmacy) | en |
| dc.date.accessioned | 2011-04-11T19:56:01Z | |
| dc.date.available | 2011-04-11T19:56:01Z | |
| dc.date.issued | 2011-04-11T19:56:01Z | |
| dc.degree.discipline | Pharmacy | en_US |
| dc.degree.level | Doctor of Philosophy (Ph.D.) | en_US |
| dc.description.abstract | Background: Multiple sclerosis (MS) is a chronic, neurological disease characterized by targeted destruction on central nervous system (CNS) myelin. The autoimmune theory is the most widely accepted explanation of disease pathology. Circulating Th-1 cells become activated by exposure to CNS-specific antigens such as myelin basic protein. The activated Th-1 cells secrete inflammatory cytokines, which are pivotal for inflammatory responses. We hypothesize that enhanced production of inflammatory cytokines triggers cellular events within the dorsal root ganglia (DRG) and/or spinal cord, facilitating the development of neuropathic pain (NPP) in MS. NPP, the second worst disease-induced symptom suffered by patients with MS, is normally regulated by DRG and/or spinal cord. Objective: To determine gene and protein expression levels of tumor necrosis factor-alpha (TNF ) within DRG and/or spinal cord in an animal model of MS. Methods: Experimental autoimmune encephalomyelitis (EAE) was induced in adolescent female Lewis rats. Animals were sacrificed every 3 days post-disease induction. DRG and spinal cords were harvested for protein and gene expression analysis. Results: We show significant increases in TNF expression in the DRG and of EAE animals at peak disease stage, as assessed by clinical symptoms. Conclusion: Antigen-induced production of inflammatory cytokines such as TNF within the DRG identifies a potential noel mechanism for MS-induced NPP. | en |
| dc.description.note | May 2011 | en |
| dc.format.extent | 11160176 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.citation | Melanson, Maria (2008). Axotomy-induced up-regulation of tumor necrosis factor-alpha in the dorsal root ganglia. Neurol Res. Jul;30(6):623-31, Epub 2008 May 16. | en |
| dc.identifier.uri | http://hdl.handle.net/1993/4516 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | TNS alpha, tumor necorsis factor, dorsal root ganglia, EAE | en |
| dc.title | NeuroImmune modulation of multiple sclerosis via the dorsal root ganglia | en |
| dc.type | doctoral thesis | en_US |