Identification of a novel TetR-family transcription regulator, PsrA, and its involvement in Legionella pneumophila virulence

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dc.contributor.authorPatel, Palak
dc.contributor.examiningcommitteeHausner, Georg (Microbiology) McKenna, Sean (Chemistry)en_US
dc.contributor.supervisorBrassinga, Ann Karen C.(Microbiology)en_US
dc.date.accessioned2014-08-18T16:28:13Z
dc.date.available2014-08-18T16:28:13Z
dc.date.issued2014-08-18
dc.degree.disciplineMicrobiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractLegionella pneumophila, an intracellular pathogen of protozoa, is well known for its dimorphic life cycle that alternates between the vegetative replicative form (RF) and highly infectious cyst-like form (CLF). To this date several virulence factors including LpRpoS, LpIHF, and the Dot/Icm secretion system have been found to be required for the survival of L. pneumophila in macrophage and protozoa. Here we have identified and characterised Lpg1967, an orthologue of Pseudomonas PsrA in L. pneumophila. PsrA (Lpg1967) was found to regulate the expression of previously known virulence factors such non-coding RNAs, RsmY/Z, RpoS, LpIHF, flagella and Dot/Icm Type IV secretion system. In addition, the ΔpsrA mutant strain was unable to establish Legionella-containing vacuole and thus displayed a severe growth defect in the U937 derived macrophage cell line. Thus, PsrA was found to play an important role in controlling the regulatory cascade governing virulence in L. pneumophila.en_US
dc.description.noteOctober 2014en_US
dc.identifier.urihttp://hdl.handle.net/1993/23813
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectLegionellaen_US
dc.subjectPsrAen_US
dc.subjecttranscription factoren_US
dc.subjectfootprintingen_US
dc.subjectDNA/protein interaction studiesen_US
dc.subjectmolecular geneticsen_US
dc.titleIdentification of a novel TetR-family transcription regulator, PsrA, and its involvement in Legionella pneumophila virulenceen_US
dc.typemaster thesisen_US
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