Human milk storage conditions in regard to safety and optimal preservation of nutritional properties

Thumbnail Image
Abramovich, Milana
Journal Title
Journal ISSN
Volume Title
This study assessed losses of the linoleic, alpha-linolenic, arachidonic and docosahexaenoic fatty acids, vitamin B2 and total vitamin C during storage of human milk, pasteurized and unpasteurized. The volatile compounds’ pattern change was monitored. The storage conditions were 8 days at 4oC and 6 months at -20oC and at -80oC, with and without limitation of oxygen. Fatty acids were analyzed by GC-FID; vitamins - by HPLC; volatile compounds’ concentration patterns were obtained using the e-nose machine; ANOVA tests were applied, with the statistical significance assigned to P<0.05. The official recommendations for human milk storage of 5-8 days at 4oC and of 6 or more months at -20oC are appropriate in regard to the analyzed nutrients and can be extended for pasteurized human milk storage. Oxygen limitation and -80oC temperature have no clear benefit for nutrient preservation. The evaluation of the odour cannot be based exclusively on the e-nose analysis.
breastmilk, storage, nitrogen, pasteurization, milk