Effects of Different Surface Expression of the CD40 Co-stimulatory Molecules on Dendritic Cell Functions
| dc.contributor.author | Zhang, Liang | |
| dc.contributor.examiningcommittee | Jude, Uzonna (Immunology) Xi, Yang (Medical Microbilogy) | en |
| dc.contributor.supervisor | Sam, Kung (Immunology) | en |
| dc.date.accessioned | 2010-04-14T14:08:21Z | |
| dc.date.available | 2010-04-14T14:08:21Z | |
| dc.date.issued | 2010-04-14T14:08:21Z | |
| dc.degree.discipline | Immunology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Dendritic cell is one of the professional antigen presenting cells, and it bridges innate immunity and adaptive immunity. To fully activate naïve T cells, it requires DC to provide at least two signals, the interaction between T cell receptor and the MHC class II molecule loaded with antigen processed by DC, and the co-stimulatory signals provided by the co-stimulatory molecules expressed on DC. The identification of more and more co-stimulatory molecules expressed on DC and the studies on their functions highlight the importance of co-stimulatory molecules on the regulation of DC functions. We here hypothesized that different expression levels of co-stimulatory molecules expressed on DC is pivotal of directing DC function towards immunity, tolerance and polarization of Th1/Th2 immune response. Using CD40 as the model molecule to study the effect of its expression levels on DC functions, we found that no/low expression level of CD40 on DC induced antigen-specific immunological tolerance was due to the induction of CD4+CD25+Foxp3+ regulatory T cells, while the polarization of Th2 immune response induced by DC with medium expression level of CD40 was partially due to the impaired IL-12 production by DC during CD40 crosslinking. Our findings that different levels of co-stimulatory molecules have different regulations on DC functions has the significance in DC based immunotherapy for GVHD as well as the Th1 diseases. | en |
| dc.description.note | May 2010 | en |
| dc.format.extent | 1030168 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.citation | Liang, Zhang (2009). Functional analysis of the quantitative expression of a costimulatory molecule on dendritic cells using lentiviral vector-mediated RNA interference, J Immunol Methods 344(2):87-97 | en |
| dc.identifier.uri | http://hdl.handle.net/1993/3982 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | DC | en |
| dc.subject | CD40 | en |
| dc.title | Effects of Different Surface Expression of the CD40 Co-stimulatory Molecules on Dendritic Cell Functions | en |
| dc.type | master thesis | en_US |