The three-dimensional (3D) organization of telomeres during cellular transformation

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dc.contributor.authorChuang, Tony Chih-Yuan
dc.contributor.examiningcommitteeKerr, Paul (Otolaryngology Head and Neck Surgery) Davie, James (Physiology) Kroeger, Edwin (Physiology) Shiu, Robert (Physiology)en
dc.contributor.supervisorMai, Sabine (Physiology)en
dc.date.accessioned2010-09-22T16:44:30Z
dc.date.available2010-09-22T16:44:30Z
dc.date.issued2010-09-22T16:44:30Z
dc.degree.disciplinePhysiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractStatement of Problem Telomere dynamics in the three-dimensional (3D) space of the mammalian nucleus plays an important role in the maintenance of genomic stability. However, the telomere distribution in 3D nuclear space of normal and tumor cells was unknown when the study was initiated. Methods Telomere fluorescence in situ hybridization (FISH) and 3D molecular imaging, deconvolution, and analysis were used to investigate telomere organization in normal, immortalized and tumor cells from mouse and human cell lines, and primary tissues. Results Telomeres are organized in a non-overlapping manner and in a cell-cycle dependant fashion in normal cells. In the late G2 phase of cell cycle, telomeres are assembled into a flattened sphere that is termed the telomeric disk In contrast, the telomeric disk is disrupted in the tumor cells. Moreover, telomeric aggregates (TAs) are found in tumor cells. Conditional c-Myc over-expression induces telomeric aggregation leading to the onset of breakage-bridge-fusion cycles and subsequent chromosomal abnormality. Conclusions Telomeres are distributed in a nonrandom and dynamic fashion in the 3D space of a normal cell. Telomeric aggregates are present in cells with genomic instability such as tumor cells and cells with deregulation of c-Myc. Consequently, TA can be a useful biomarker for research in cancer and other disease processes.en
dc.description.noteOctober 2010en
dc.format.extent11076608 bytes
dc.format.extent1840301 bytes
dc.format.mimetypevideo/avi
dc.format.mimetypeapplication/pdf
dc.identifier.citationChuang, TC (2004). The three-dimensional organization of telomeres in the nucleus of mammalian cells, BMC Biol. 2004 Jun 3;2:12.en
dc.identifier.citationLouis, SF (2005). c-Myc induces chromosomal rearrangements through telomere and chromosome remodeling in the interphase nucleus. Proc Natl Acad Sci U S A. 2005 Jul 5;102(27):9613-8.en
dc.identifier.urihttp://hdl.handle.net/1993/4228
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjecttelomereen
dc.subjectc-mycen
dc.subjectdeconvolutionen
dc.subjectmolecular imagingen
dc.subjectgenomic instabilityen
dc.subjectbiomarkeren
dc.titleThe three-dimensional (3D) organization of telomeres during cellular transformationen
dc.typemaster thesisen_US
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