Electrophysiological characterization of catecholamine-containing GFP-expressing dissociated mouse area postrema neurons and their response to glucagon-like peptide-1 receptor agonists

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dc.contributor.authorLee, Samantha
dc.contributor.authorFry, Mark
dc.contributor.examiningcommitteeHare, James (Biological Sciences) Kirouac, Gilbert (Oral Biology)en_US
dc.contributor.supervisorFry, Mark (Biological Sciences)en_US
dc.date.accessioned2017-09-07T16:42:31Z
dc.date.available2017-09-07T16:42:31Z
dc.date.issued2017
dc.degree.disciplineBiological Sciencesen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractThe area postrema (AP) is a hindbrain sensory circumventricular organ that participates in energy balance regulation. Catecholamine-containing AP neurons are a key subpopulation that inhibit food intake and appear to mediate the appetite-reducing effects of glucagon-like peptide-1 (GLP-1) receptor agonists. Neither the electrophysiological properties nor the electrical response of CA-containing neurons to GLP-1 receptor agonists has been characterized. Therefore, we carried out patch clamp experiments on dissociated neuronal cultures from transgenic mice (TH-GFP). Voltage-clamp recordings revealed subtle differences in K+ current, Na+ current, and IH properties between TH and nonTH neurons. In current-clamp configuration, application of 1 μM GLP-1 or 1 μM Exendin-4 activated the majority of TH neurons tested. Our results suggest that TH neurons are an electrophysiologically heterogeneous population and confirm that GLP-1 receptor agonists activate most TH neurons. Together, it appears that TH neurons play multiple roles in integrating satiety information to ultimately reduce food intake.en_US
dc.description.noteOctober 2017en_US
dc.identifier.urihttp://hdl.handle.net/1993/32494
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectElectrophysiologyen_US
dc.subjectGLP-1en_US
dc.subjectArea postremaen_US
dc.subjectTyrosine hydroxylaseen_US
dc.titleElectrophysiological characterization of catecholamine-containing GFP-expressing dissociated mouse area postrema neurons and their response to glucagon-like peptide-1 receptor agonistsen_US
dc.typemaster thesisen_US
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