Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression

Simple item page
dc.contributor.authorDelgado-Diaz, David J.
dc.contributor.authorJesaveluk, Brianna
dc.contributor.authorHayward, Joshua A.
dc.contributor.authorTyssen, David
dc.contributor.authorAlisoltani, Arghavan
dc.contributor.authorPotgieter, Matthys
dc.contributor.authorBell, Liam
dc.contributor.authorRoss, Elizabeth
dc.contributor.authorIranzadeh, Arash
dc.contributor.authorAllali, Imane
dc.contributor.authorDabee, Smritee
dc.contributor.authorBarnabas, Shaun
dc.contributor.authorGamieldien, Hoyam
dc.contributor.authorBlackburn, Jonathan M.
dc.contributor.authorMulder, Nicola
dc.contributor.authorSmith, Steven B.
dc.contributor.authorEdwards, Vonetta L.
dc.contributor.authorBurgener, Adam D.
dc.contributor.authorBekker, Linda-Gail
dc.contributor.authorRavel, Jacques
dc.contributor.authorPassmore, Jo-Ann S.
dc.contributor.authorMasson, Lindi
dc.contributor.authorHearps, Anna C.
dc.contributor.authorTachedjian, Gilda
dc.date.accessioned2022-09-01T03:20:38Z
dc.date.issued2022-08-31
dc.date.updated2022-09-01T03:20:38Z
dc.description.abstractAbstract Background Women with a cervicovaginal microbiota dominated by Lactobacillus spp. are at reduced risk of acquiring sexually transmitted infections including HIV, but the biological mechanisms involved remain poorly defined. Here, we performed metaproteomics on vaginal swab samples from young South African women (n = 113) and transcriptomics analysis of cervicovaginal epithelial cell cultures to examine the ability of lactic acid, a metabolite produced by cervicovaginal lactobacilli, to modulate genital epithelial barrier function. Results Compared to women with Lactobacillus-depleted microbiota, women dominated by vaginal lactobacilli exhibit higher abundance of bacterial lactate dehydrogenase, a key enzyme responsible for lactic acid production, which is independently associated with an increased abundance of epithelial barrier proteins. Physiological concentrations of lactic acid enhance epithelial cell culture barrier integrity and increase intercellular junctional molecule expression. Conclusions These findings reveal a novel ability of vaginal lactic acid to enhance genital epithelial barrier integrity that may help prevent invasion by sexually transmitted pathogens. Video abstract
dc.identifier.citationMicrobiome. 2022 Aug 31;10(1):141
dc.identifier.urihttps://doi.org/10.1186/s40168-022-01337-5
dc.identifier.urihttp://hdl.handle.net/1993/36833
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderThe Author(s)
dc.titleLactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression
dc.typeJournal Article
local.author.affiliationRady Faculty of Health Sciencesen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
40168_2022_Article_1337.pdf
Size:
3.17 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.24 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
University of Manitoba Scholarship
Rady Faculty of Health Sciences Scholarly Works