The role(s) of JLP scaffolding protein in regulating LPS- vs. poly(I:C)-activated mature dendritic cell functions
| dc.contributor.author | Zhao, Chongbo | |
| dc.contributor.examiningcommittee | Soussi Gounni, Abdelilah (Immunology) Xie, Jiuyong (Physiology) | en_US |
| dc.contributor.supervisor | Kung, Sam (Immunology) | en_US |
| dc.date.accessioned | 2012-12-20T17:57:25Z | |
| dc.date.available | 2012-12-20T17:57:25Z | |
| dc.date.issued | 2012-12-20 | |
| dc.degree.discipline | Immunology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | TLR3- and TLR4-mediated DC activation lead to distinct function of mature DC, in which MAPKs pathway is involved. JNK-associated leucine zipper protein (JLP) associates with JNK/p38 phosphorylation, however the role of JLP in DC is not well understood. We previously found either LPS or poly(I:C) up-regulated JLP expression in BMDC. Therefore we hypothesized JLP plays a differential role in TLR3- and TLR4-mediated BMDC maturation and functions. We demonstrated JLP facilitated LPS- and poly(I:C)-induced CD86 and CD40 up-regulation, LPS-induced CD80 up-regulation and poly(I:C)-induced MHC-II up-regulation. We found JLP down-regulated IL-12 production in LPS-stimulated BMDC, and up-regulated IL-6 production in poly(I:C)-stimulated BMDC. Our data also showed JLP negatively regulated MHC-II antigen presentation in LPS- and poly(I:C)-stimulated BMDC and JLP was involved in promoting LPS-activated BMDC survival, but not in poly(I:C)-activated BMDCs. Therefore our current data suggested a multi-functional role of JLP in the regulation of TLR3- and TLR4-mediated DC maturation. | en_US |
| dc.description.note | February 2013 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/14165 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Dendritic cell | en_US |
| dc.subject | Toll-like receptor | en_US |
| dc.subject | Scaffold protein | en_US |
| dc.subject | JLP | en_US |
| dc.title | The role(s) of JLP scaffolding protein in regulating LPS- vs. poly(I:C)-activated mature dendritic cell functions | en_US |
| dc.type | master thesis | en_US |