Effect of hemp seed protein isolate on mesenteric resistance artery isolated from the spontaneously hypertensive rat

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dc.contributor.authorParaplammoottil Cheriyan, Sherin
dc.contributor.examiningcommitteeAluko, Rotimi (Food and Human Nutritional Sciences)en_US
dc.contributor.examiningcommitteeSuh, Miyoung (Food and Human Nutritional Sciences)en_US
dc.contributor.supervisorAnderson, Hope
dc.contributor.supervisorBlewett, Heather
dc.date.accessioned2022-08-15T19:23:14Z
dc.date.available2022-08-15T19:23:14Z
dc.date.copyright2022-08-11
dc.date.issued2022-08-11
dc.date.submitted2022-08-11T21:04:01Zen_US
dc.degree.disciplinePharmacyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractHypertensive vessels are characterized by attenuated endothelium-dependent vascular relaxation or endothelial dysfunction. Hemp seed protein isolate (HPI) is a novel protein that contains more than 80% of crude hemp protein and exhibits antihypertensive properties. In addition, HPI contains high levels of arginine, a pre-cursor for vasodilator nitric oxide (NO). However, it is unknown whether HPI can improve the vascular function of hypertensive vessels. Also, sex differences are observed in vascular function and BP regulation, with males at greater risk as opposed to age-matched pre-menopausal women. The present study tested the hypothesis that direct treatment of HPI will lead to vascular relaxation of pre-constricted mesenteric resistance arteries. In addition, we also addressed sex differences in vascular response to HPI. To investigate the potential vasorelaxant activity, we tested HPI (0.1 to 100 µg/mL) on third-order mesenteric arteries using the pressure myography technique. We found that HPI relaxed mesenteric resistance arteries isolated from 16-week-old male and female Sprague Dawley (SD), spontaneously hypertensive rat (SHR), and Wistar-Kyoto (WKY) rats in a dose-dependent dependent manner. The vasorelaxant activity of HPI is probably due to the presence of amino acids such as arginine, cysteine, and serine. Also, male SD rats showed significantly lower %dilation compared to female SD rats. Whereas male WKY showed significantly higher HPI induced %dilation response compared to female WKY, female SHR, and male SHR. Sex differences observed in normotensive rats are probably due to bioactive components of HPI. Further studies are required to identify the mechanism involved in HPI-induced vasodilation and elucidate the sex difference observed. In summary, we have shown for the first time that HPI is a vasodilator. Our study findings indicate that HPI may be used for improving the vascular function of hypertensive vessels.en_US
dc.description.noteOctober 2022en_US
dc.description.sponsorshipCollege of Pharmacy Targeted Donor Supporten_US
dc.identifier.urihttp://hdl.handle.net/1993/36685
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjecthemp seed protein isolateen_US
dc.subjectspontaneously hypertensive ratsen_US
dc.subjecthypertensionen_US
dc.subjectvascular functionen_US
dc.subjectmesenteric resistance arteryen_US
dc.titleEffect of hemp seed protein isolate on mesenteric resistance artery isolated from the spontaneously hypertensive raten_US
dc.typemaster thesisen_US
local.subject.manitobanoen_US
project.funder.nameNot applicableen_US
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