Genetic characterization of Canadian group A human rotavirus strains collected in multiple paediatric hospitals from 2007-2010
| dc.contributor.author | McDermid, Andrew | |
| dc.contributor.examiningcommittee | Embree, Joanne (Medical Microbiology) Coombs, Kevin (Medical Microbiology) Crockett, Maryanne (Medical Microbiology) Bhullar, Raj (Oral Biology) | en_US |
| dc.contributor.supervisor | Booth, Tim (Medical Microbiology) | en_US |
| dc.date.accessioned | 2012-08-28T19:51:43Z | |
| dc.date.available | 2012-08-28T19:51:43Z | |
| dc.date.issued | 2012-08-28 | |
| dc.degree.discipline | Medical Microbiology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Group A rotaviruses are a major cause of acute gastroenteritis in children. Almost all children are infected by the age of 5 years old. Rotavirus disease causes around 600,000 deaths per year. VP4 (P) and VP7 (G) genotypes were analyzed for prevalence and potential antigenicity, as they are known to elicit a neutralizing antibody response during infection. This study predicted the effectiveness of two recently licensed rotavirus vaccines based on Canadian surveillance. 271 out of 348 diarrhea samples from 8 paediatric hospital were successfully genotyped by PCR. Canadian rotavirus genotypes were found to be mostly G1P[8] followed by G3P[8], G2P[4], G9P[8], G4P[8], and G9P[4], between 2007 and 2010. Reassortment and motif analysis was done with a subset of rotavirus-positive samples. There were no unusual reassortment events found in Canadian strains. Variations amongst strains were commonly genotype-specific, but otherwise rare. In conclusion, rotavirus vaccine escape is presently unlikely amongst Canadian strains. | en_US |
| dc.description.note | October 2012 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/8581 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Rotavirus | en_US |
| dc.subject | Genotyping | en_US |
| dc.subject | Genetics | en_US |
| dc.subject | Phylogenetics | en_US |
| dc.subject | PCR | en_US |
| dc.subject | Real-Time PCR | en_US |
| dc.subject | Pediatric | en_US |
| dc.subject | Diarrhea | en_US |
| dc.subject | Gastroenteritis | en_US |
| dc.subject | IMPACT | en_US |
| dc.title | Genetic characterization of Canadian group A human rotavirus strains collected in multiple paediatric hospitals from 2007-2010 | en_US |
| dc.type | master thesis | en_US |