A (H1N1) pdm09 HA D222 variants associated with severity and mortality in patients during a second wave in Mexico

Simple item page
dc.contributor.authorVazquez-Perez, Joel A
dc.contributor.authorIsa, Pavel
dc.contributor.authorKobasa, Darwyn
dc.contributor.authorOrmsby, Christopher E
dc.contributor.authorRamirez-Gonzalez, Jose E
dc.contributor.authorRomero-Rodriguez, Damaris P
dc.contributor.authorRanadheera, Charlene
dc.contributor.authorLi, Yan
dc.contributor.authorBastien, Nathalie
dc.contributor.authorEmbury-Hyatt, Carissa
dc.contributor.authorGonzalez-Duran, Elizabeth
dc.contributor.authorBarrera-Badillo, Gisela
dc.contributor.authorAblanedo-Terrazas, Yuria
dc.contributor.authorSevilla-Reyes, Edgar E
dc.contributor.authorEscalera-Zamudio, Marina
dc.contributor.authorCobian-Guemes, Ana G
dc.contributor.authorLopez, Irma
dc.contributor.authorOrtiz-Alcantara, Joanna
dc.contributor.authorAlpuche-Aranda, Celia
dc.contributor.authorPerez-Padilla, Jose R
dc.contributor.authorReyes-Teran, Gustavo
dc.date.accessioned2013-02-27T16:04:43Z
dc.date.available2013-02-27T16:04:43Z
dc.date.issued2013-01-31
dc.date.updated2013-02-27T16:04:43Z
dc.description.abstractAbstract Background Pandemic type A (H1N1) influenza arose in early 2009, probably in Mexico and the United States, and reappeared in North America in September for seven more months. An amino acid substitution in the hemagglutinin (HA), D222G, has been reported in a significant proportion of patients with a severe and fatal outcome. We studied the prevalence of HA222 substitutions in patients in Mexico during the second wave and its association with clinical outcome and pathogenicity in a mouse model. Methods The nucleotide sequences of hemagglutinin (HA) from viruses collected from 77 patients were determined including 50 severe and fatal cases and 27 ambulatory cases. Deep sequencing was done on 5 samples from severe or fatal cases in order to determine the quasispecies proportion. Weight loss and mortality due to infection with cultured influenza viruses were analyzed in a mouse model. Results Viruses from 14 out of 50 hospitalized patients (28%) had a non aspartic acid residue at the HA 222 position (nD222), while all 27 ambulatory patients had D222 (pā€‰=ā€‰0.0014). G222 was detected as sole species or in coexistence with N222 and D222 in 12 patients with severe disease including 8 who died. N222 in coexistence with D222 was detected in 1 patient who died and co-occurrence of A222 and V222, together with D222, was detected in another patient who died. The patients with a nD222 residue had higher mortality (71.4%), compared to the group with only D222 (22.2%, pā€‰=ā€‰0.0008). Four of the 14 viruses from hospitalized patients were cultured and intranasally infected into mice. Two viruses with G222 were lethal while a third virus, with G222, caused only mild illness in mice similar to the fourth virus that contained D222. Conclusions We confirm the elevated incidence of HA222 (H1N1)pdm09 variants in severe disease and mortality. Both clinical and mouse infection data support the idea that nD222 mutations contribute to increased severity of disease but additional determinants in disease outcome may be present.
dc.description.versionPeer Reviewed
dc.identifier.citationVirology Journal. 2013 Jan 31;10(1):41
dc.identifier.doihttp://dx.doi.org/10.1186/1743-422X-10-41
dc.identifier.urihttp://hdl.handle.net/1993/16850
dc.language.rfc3066en
dc.rightsopen accessen_US
dc.rights.holderJoel A Vazquez-Perez et al.; licensee BioMed Central Ltd.
dc.titleA (H1N1) pdm09 HA D222 variants associated with severity and mortality in patients during a second wave in Mexico
dc.typeJournal Article
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
1743-422X-10-41.xml
Size:
103.58 KB
Format:
Extensible Markup Language
Description:
Download
Thumbnail Image
Name:
1743-422X-10-41.pdf
Size:
3.38 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
University of Manitoba Scholarship
Rady Faculty of Health Sciences Scholarly Works