Detection of nonstructural protein NSm1 in Rift Valley Fever Virus virions assembled in insect but not mammalian cells
| dc.contributor.author | McGreevy, Alan | |
| dc.contributor.examiningcommittee | Coombs, Kevin (Medical Microbiology) Wrogemann, Klaus (Biochemistry and Medical Genetics) | en_US |
| dc.contributor.supervisor | Weingartl, Hana (Medical Microbiology) | en_US |
| dc.date.accessioned | 2013-01-07T17:26:51Z | |
| dc.date.available | 2013-01-07T17:26:51Z | |
| dc.date.issued | 2013-01-07 | |
| dc.degree.discipline | Medical Microbiology | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Rift Valley fever virus is a zoonotic pathogen that is transmitted between mosquitoes and mammals such as sheep, cattle and humans. It is an enveloped negative-sense single-stranded RNA virus, which is a member of the family Bunyaviridae and the genus Phlebovirus. During replication, RVFV produces a 78 kDa glycoprotein NSm1 of unknown function, believed to be nonstructural. Here I show that NSm1 is incorporated into RVFV virions assembled in C6/36 mosquito cells, but not in virions assembled in Vero E6 mammalian cells. The presence of NSm1 in insect-amplified virions was demonstrated through repeated immunoblots of purified virions and further supported by mass spectrophotometric confirmation of the identity of immunoblot-positive protein bands. This research appears to be the first evidence that distinct viral protein profiles are correlated to the host cell in which replication occurred. | en_US |
| dc.description.note | February 2013 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/14413 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | Arbovirus | en_US |
| dc.title | Detection of nonstructural protein NSm1 in Rift Valley Fever Virus virions assembled in insect but not mammalian cells | en_US |
| dc.type | master thesis | en_US |