Co-toxicity of ATP and menadione, effects on intracellular calcium regulation in hepatocytes
| dc.contributor.author | Fisher, Candace E. | en_US |
| dc.date.accessioned | 2007-05-18T19:59:16Z | |
| dc.date.available | 2007-05-18T19:59:16Z | |
| dc.date.issued | 1999-08-01T00:00:00Z | en_US |
| dc.degree.discipline | Pharmacology and Therapeutics | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | The effects of non-lethal oxidative stress on cytosolic calcium regulation in hepatocytes are inadequately understood. It was hypothesized that non-lethal oxidative stress and calcium mobilizing agonists are co-toxic and that non-lethal oxidative injury would result in impaired responsiveness to receptor mediated-calcium mobilization. 'Methods'. Oxidative injury was induced in a dose-dependent manner in cultured hepatocytes using the prooxidant chemical, menadione. A lethal (100[mu]M) and a non-lethal (10[mu]M) dose of menadione were defined. Co-toxicity of the calcium-mobilizing purinergic receptor agonist, ATP, and the effects of menadione on ATP-related calcium signaling were determined by incubating hepatocytes for 2 hours with menadione and then exposing hepatocytes to normally non-toxic concentrations of ATP. A propidium iodide exclusion assay was used to assess for cell necrosis. Nuclear stains with Hoechst 33258 and a fluorescein-based TUNEL assay were used to assess apoptosis. Cytosolic free calcium was evaluated using Fura-2-AM. (Abstract shortened by UMI.) | en_US |
| dc.format.extent | 2586126 bytes | |
| dc.format.extent | 184 bytes | |
| dc.format.mimetype | application/pdf | |
| dc.format.mimetype | text/plain | |
| dc.identifier.uri | http://hdl.handle.net/1993/1803 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.title | Co-toxicity of ATP and menadione, effects on intracellular calcium regulation in hepatocytes | en_US |
| dc.type | master thesis | en_US |