Suppression of the soybean phytoglobin GmPgb1 enhances tolerance to iron deficiency

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dc.contributor.authorAsmundson, Bethany
dc.contributor.examiningcommitteeHill, Robert (Plant Science) Renault, Sylvia (Biological Sciences)en_US
dc.contributor.supervisorStasolla, Claudio (Plant Science)en_US
dc.date.accessioned2021-01-16T19:59:04Z
dc.date.available2021-01-16T19:59:04Z
dc.date.copyright2021-01-02
dc.date.issued2020en_US
dc.date.submitted2021-01-02T22:56:34Zen_US
dc.degree.disciplinePlant Scienceen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractThe effect of iron deficiency in soybean (Glycine max (L.) merr) seedlings over-expressing or down-regulating Glycine max Phytoglobin 1 (GmPgb1) was examined using an established hydroponic system which mimicked iron deficiency in soil. Iron deficient soybean plants down-regulating GmPgb1 displayed greater chlorophyll retention and decreased accumulation of reactive oxygen species relative to the GmPbg1 over-expressing and wild type (WT) plants. Suppression of GmPgb1 under iron deficiency conditions was also accompanied by elevated levels of ascorbic acid (ASC), and increased activities of catalase, superoxide dismutase and ASC recycling enzymes. When compared to WT and GmPgb1 over-expressing lines, iron deficient GmPgb1 suppressing plants also exhibited a higher Fe(II)/Fe(III) ratio. Pharmacological treatments altering the level of nitric oxide (NO) and ASC were further utilized to dissect the effects of GmPgb1 during iron deficiency. Applications of the NO donor, sodium nitroprusside (SNP), enhanced tolerance to iron deficiency by elevating the levels of ASC, the activities of several antioxidant enzymes, and the Fe(II)/Fe(III) ratio. A similar effect on this ratio was also observed when ASC was administered exogenously. Collectively this study reveals that suppression of GmPgb1 in soybean attenuates the effects of iron deficiency through changes in NO and antioxidant responses linked to the higher availability of Fe(II).en_US
dc.description.noteFebruary 2021en_US
dc.identifier.urihttp://hdl.handle.net/1993/35246
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectIron deficiencyen_US
dc.subjectPhytoglobinen_US
dc.subjectAscorbic aciden_US
dc.subjectSoybeanen_US
dc.subjectNitric oxideen_US
dc.subjectGlycine maxen_US
dc.titleSuppression of the soybean phytoglobin GmPgb1 enhances tolerance to iron deficiencyen_US
dc.typemaster thesisen_US
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