Differential roles of RNA binding proteins hnRNP L and LL in hormone production in rat pituitary cells

Simple item page
dc.contributor.authorLei, Lei
dc.contributor.examiningcommitteeMizuno, Tooru (Physiology and Pathophysiology) Myal, Yvonne (Pathology) Kung, Sam (Immunology)en_US
dc.contributor.supervisorXie, Jiuyong (Physiology and Pathophysiology)en_US
dc.date.accessioned2015-09-28T20:18:09Z
dc.date.available2015-09-28T20:18:09Z
dc.date.issued2015
dc.degree.disciplinePhysiology and Pathophysiologyen_US
dc.degree.levelMaster of Science (M.Sc.)en_US
dc.description.abstractOBJECTIVES: The role of heterogeneous nuclear ribonucleoproteins in controlling hormone production is not well-understood. This study aims to determine the regulatory roles of hnRNP L and LL in hormone production. RESULTS: HnRNP LL knockdown dramatically reduced the mRNA level of growth hormone (GH) and prolactin (PRL) in GH3 pituitary cells, which was efficiently rescued by a Myc-hnRNP LL. In contrast, hnRNP L knockdown caused a 93-nucleotide intron retention with a decrease in the full-length Prl, which was rescued by a FLAG-hnRNP L. Double knockdown of hnRNPs indicates the interactions of hnRNPs for particular effects. The essential role of hnRNP LL for the secretion of GH and PRL was verified in primary pituitary cultures. Using RNA sequencing, a group of genes in hormone production that were differentially regulated by hnRNP L or LL were identified. CONCLUSION: These findings demonstrate the differential functions of two paralogous RNA processing factors in hormone production.en_US
dc.description.noteFebruary 2016en_US
dc.identifier.urihttp://hdl.handle.net/1993/30848
dc.language.isoengen_US
dc.rightsopen accessen_US
dc.subjectEndocrineen_US
dc.subjectHormonesen_US
dc.subjectRNAen_US
dc.subjectSplicingen_US
dc.subjectProcessingen_US
dc.subjectGene regulationen_US
dc.subjectGene expressionen_US
dc.subjectMolecular biologyen_US
dc.titleDifferential roles of RNA binding proteins hnRNP L and LL in hormone production in rat pituitary cellsen_US
dc.typemaster thesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Lei_Lei.pdf
Size:
3.36 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.25 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
FGS - Electronic Theses and Practica