Investigating the relationship between the immune response and ependymoglial activation during spinal cord regeneration in the adult and juvenile zebrafish model
| dc.contributor.author | Trzuskot, Lidia | |
| dc.contributor.examiningcommittee | Karimi, Soheila (Physiology and Pathophysiology) | en_US |
| dc.contributor.examiningcommittee | Logue, Susan (Human Anatomy and Cell Science) | en_US |
| dc.contributor.supervisor | Lindsey, Benjamin | |
| dc.date.accessioned | 2022-04-05T15:07:37Z | |
| dc.date.available | 2022-04-05T15:07:37Z | |
| dc.date.copyright | 2022-03-29 | |
| dc.date.issued | 2022-03-29 | |
| dc.date.submitted | 2022-03-29T21:53:15Z | en_US |
| dc.degree.discipline | Human Anatomy and Cell Science | en_US |
| dc.degree.level | Master of Science (M.Sc.) | en_US |
| dc.description.abstract | Spinal cord injury (SCI) is a life changing condition affecting individuals within Canada and worldwide with no effective treatment to date. A limitation in humans, like other mammals, is that they cannot repair the damaged central nervous system after injury. By contrast, the zebrafish model has a remarkable ability to regenerate the spinal cord following complete transection, due to neural stem cell populations of ependymoglia. Previous work has shown that for ependymoglial-driven neural regeneration to occur, immune cells are a key requirement. However, in zebrafish the involvement of macrophages and the cytokine response during the process of spinal cord regeneration in post-larval stages remains poorly understood. In this study, I hypothesized that for functional recovery to occur, the pro-inflammatory response following SCI in zebrafish must be activated ahead of ependymoglial proliferation to initiate the regenerative process. To study this response, I developed a new juvenile model of SCI to then compare to the established adult model of SCI. By studying the spatiotemporal dynamics of immune cells post-SCI, I observed that overtime macrophages and microglia infiltrate into the injury site and contributed to cytokine release, correlating with a peak in proliferation of ependymoglia around the central canal. Interestingly, analysis of pro- and anti-inflammatory cytokines from RT-qPCR experiments demonstrated that pro-inflammatory cytokines are highly expressed shortly after injury, but are reduced to near control levels already by 3-days post-SCI. By contrast, anti-inflammatory cytokines appeared to play a minor role in the microenvironment post-SCI, remaining near control levels of expression. These findings propose that in order for successful spinal cord regeneration to occur in adult and juvenile zebrafish, a shorter pro-inflammatory response may be required to initiate ependymoglial proliferation in the spinal cord and restore functional repair. | en_US |
| dc.description.note | May 2022 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1993/36398 | |
| dc.language.iso | eng | en_US |
| dc.rights | open access | en_US |
| dc.subject | zebrafish | en_US |
| dc.subject | spinal cord injury | en_US |
| dc.subject | neuroregeneration | en_US |
| dc.title | Investigating the relationship between the immune response and ependymoglial activation during spinal cord regeneration in the adult and juvenile zebrafish model | en_US |
| dc.type | master thesis | en_US |
| project.funder.name | Manitoba Medical Service Foundation | en_US |